+ Translate

Evaluation of a heparin assay method using a fluorogenic synthetic peptide substrate for thrombin

, : Evaluation of a heparin assay method using a fluorogenic synthetic peptide substrate for thrombin. Thrombosis Research 25(1-2): 115-124

A heparin assay method based on thrombin cleavage of a fluorogenic synthetic peptide was evaluated in normal subjects, in patients with chronic renal disease on hemodialysis and in patients with other disorders. The method has satisfactory accuracy, sensitivity and reproducibility. In contrast to methods commonly used to monitor heparin therapy, it measures heparin directly and is uninfluenced by patient variables such as low antithrombin III. The method may provide valuable information in selected clinical settings such as cardiopulmonary bypass and in patients with chronic renal disease who receive heparin during hemodialysis or prior to surgery.

Accession: 005395141

PMID: 7064128

DOI: 10.1016/0049-3848(82)90220-1

Download PDF Full Text: Evaluation of a heparin assay method using a fluorogenic synthetic peptide substrate for thrombin

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Suzuki, K.; Nishioka, J.; Hashimoto, S., 1980: Assay method of plasma plasminogen by means of a synthetic fluorogenic peptide substrate (3104-V). Rinsho Byori. Japanese Journal of Clinical Pathology 28(8): 804-807

Mitchell, G.A.; Gargiulo, R.J.; Huseby, R.M.; Lawson, D.E.; Pochron, S.P.; Sehuanes, J.A., 1978: Assay for plasma heparin using a synthetic peptide substrate for thrombin introduction of the fluorophore amino iso phthalic acid di methyl ester. A precise sensitive method for assay of plasma heparin anticoagulant activity using a synthetic fluorescent tripeptide, H-D-phe-pro-arg-5-amidoisophthalic acid, dimethyl ester, as a substrate for thrombin is described. The assay procedure is desig...

Pochron S.P., 1979: The use of the fluorogenic synthetic substrate assay for the clinical determination of heparin. Thrombosis & Haemostasis: 308

Galluzzo, T.S.; Ts'ao, C.H., 1982: Evaluation of a thrombin-sensitive fluorogenic substrate for heparin concentration in plasma. Heparin levels in 76 heparinized patient plasma samples were determined with a thrombin-sensitive fluorogenic substrate. Results were compared with heparin values derived from an activated partial thromboplastin time test. The correlation of hepar...

Suzuki, K.; Nishioka, J.; Hashimoto, S., 1979: Assay method for urinary urokinase using synthetic fluorogenic substrate. Rinsho Byori. Japanese Journal of Clinical Pathology 27(3): 218-222

Triplett D.A.; Harms C.; Huseby R.M.; Mitchell G.A.; Pochron S.P., 1979: Clinical studies of the use of a fluorogenic substrate assay for the determination of anti thrombin iii activity. Thrombosis & Haemostasis 42(2): 20

Geoghegan, Kieran F., 1994: Method for converting a peptide substrate of a proteinase to a fluorogenic substrate. Journal of Cellular Biochemistry Supplement 0(18D): 133

Lively, T.J.; Bosco, D.B.; Khamis, Z.I.; Sang, Q-Xiang.Amy., 2016: Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay. Matrix metalloproteinases (MMPs) are a family of metzincin enzymes that act as the principal regulators and remodelers of the extracellular matrix (ECM). While MMPs are involved in many normal biological processes, unregulated MMP activity has bee...

Backes, B.J.; Harris, J.L.; Leonetti, F.; Craik, C.S.; Ellman, J.A., 2000: Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin. We have developed a strategy for the synthesis of positional-scanning synthetic combinatorial libraries (PS-SCL) that does not depend on the identity of the P1 substituent. To demonstrate the strategy, we synthesized a tetrapeptide positional libr...

Kleijer, W.J.; Karpova, E.A.; Geilen, G.C.; Keulemans, J.L.; Huijmans, J.G.; Tsvetkova, I.V.; Voznyi YaV; van Diggelen, O.P., 1996: Prenatal diagnosis of Sanfilippo A syndrome: experience in 35 pregnancies at risk and the use of a new fluorogenic substrate for the heparin sulphamidase assay. We have investigated the use of a 4-methylumbelliferone (MU)-derived artificial substrate, MU-alpha-D-N-sulphoglucosaminide, for the sulphamidase assay in chorionic villi and amniotic fluid cells. In the new two-step enzyme assay, fluorescent MU i...