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Evaluation of a lysis direct plating method for pediatric blood cultures


, : Evaluation of a lysis direct plating method for pediatric blood cultures. Journal of Clinical Microbiology 21(6): 955-958

The Isolator 1.5 Microbial tube (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) is a commercially available blood culture system for use in pediatrics. The methodology is based on blood lysis followed by direct plating of the sample on culture media to detect bacteria and fungi. Comparative recovery rates of pathogens from blood collected in this and a conventional broth system were similar. The Isolator detected 104 of 120 clinically significant isolates, whereas 106 of 120 isolates were detected by the broth system. The major advantage of the Isolator methodology was early detection of septicemia. Initial detection of gram-negative bacteria occurred an average of 14.2 h earlier by the Isolator system than by the conventional broth method. The Isolator also permitted quantitation of bacteremia and fungemia. Probable contaminants were recovered from 10.0% of the cultures processed by the Isolator, but steps which could be taken to minimize this problem were identified. The Isolator is a useful method for pediatric blood cultures.

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Accession: 005395197

PMID: 3891774

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Related references

Welch D.F., 1983: A lysis direct plating method for pediatric blood cultures. Abstracts Of The Annual Meeting Of The American Society For Microbiology: 5

Saha, S.K.; Khan, W.A.; Saha, S., 1992: Blood cultures from Bangladeshi children with septicaemia: an evaluation of conventional, lysis-direct plating and lysis-centrifugation methods. The use of a laboratory-made lysis-direct plating and lysis-centrifugation (LDP/LC) device for blood cultivation has been compared with the conventional broth culture method in respect of speed and sensitivity in detecting organism(s) and cost eff...

Stutman, H.R.; Welch, D.F., 1985: Comparison of lysis-direct plating and broth methods for pediatric blood cultures: clinical relevance and cost effectiveness. To determine the clinical significance and therapeutic impact of pediatric blood cultures, we analyzed 1650 cultures comparing conventional broth and direct plating methods. The rate of positive cultures was 5.8%. Of 96 positive cultures 68 were d...

Pickett D.A.; Welch D.F., 1992: Clinical comparison of an automated pediatric blood culture system bact alert to lysis direct plating isolator. Abstracts of the General Meeting of the American Society for Microbiology 92: 429

Campos, J.M.; Spainhour, J.R., 1985: Rapid detection of bacteremia in children with a modified lysis direct plating method. A modified lysis direct plating method was developed for culturing blood specimens from pediatric patients. The method, which used the commercially available Isolator 1.5 Microbial Tube, consisted of direct inoculation of lysed blood to agar media...

Tison, D.L.; Latimer, J.M., 1986: Lysis-centrifugation-direct plating technique for isolation of group DF-2 from the blood of a dog bite victim. Journal of Infectious Diseases 153(5): 1001-1002

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Czaban, J.; Wroblewska, B., 2006: A simple, direct plating method, alternative to dilution plating, for estimation of the abundance of Penicillium verrucosum on incubated cereal grain. Because dilution plating is more labor intensive than direct plating, we would like to propose the use of a direct plating technique for estimation of Penicillium verrucosum abundance in cereal grain in incubation studies, with use of very select...

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