EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a membrane (Nuclepore) filtration method using capillary blood for the detection of microfilariae


Annals of Tropical Medicine and Parasitology 76(3): 339-345
Evaluation of a membrane (Nuclepore) filtration method using capillary blood for the detection of microfilariae
The efficacy of a membrane (Nuclepore) filtration technique using [human ] capillary blood (100 mm3) for the detection of blood-circulating microfilariae was investigated during a prevalence survey for Wuchereria bancrofti and Mansonella ozzardi in north Trinidad by comparing results thus obtained with results from conventional thick smear preparations (25 mm3) and from membrane (Nuclepore) filtration of venous blood (1 ml). Prevalence rates of W. bancrofti were 14% by thick smear, 17% by filtration of capillary blood and 16% by filtration of venous blood. For M. ozzardi the corresponding rates were 27, 29 and 31%. A total of 389 persons was examined by all 3 methods. Microfilaria detection rates for both of the filtration techniques were significantly higher than by thick smear preparation, with the exception of results for M. ozzardi obtained by filtration of capillary blood. There was no significant difference between the number of positive persons detected by filtration of capillary blood and filtration of venous blood. Significantly higher microfilaria densities/unit volume of blood were recorded by thick smear preparation and filtration of capillary blood than by filtration of venous blood; results for the 2 capillary blood techniques were similar. In the detection of low density microfilaremias, membrane filtration of capillary blood provides a practical and sensitive alternative to venous blood filtration and has the additional advantage of capillary blood collection rather than venepuncture.


Accession: 005395213

PMID: 6751251

DOI: 10.1080/00034983.1982.11687550



Related references

Evaluation of a membrane (Nuclepore) filtation method using capillary blood for the detection of microfilariae. Annals of tropical medicine and parasitology 76(3): 339-345, 1982

Bancroftian filariasis: a comparison of microfilariae counting techniques using counting chamber, standard slide and membrane (nuclepore) filtration. Annals of Tropical Medicine and Parasitology 73(5): 457-464, 1979

A comparison of the efficiency of the Nuclepore and Millipore filtration systems for detecting microfilariae. Southeast Asian Journal of Tropical Medicine and Public Health 7(3): 367-369, 1976

Evaluation of thick smear, Knott and membrane filtration methods for demonstrating microfilariae in blood. Tropical and Geographical Medicine 25(3): 286-289, 1973

An improved technique for the filtration of microfilariae from venous blood, and a method of recovering the microfilariae alive. Transactions of the Royal Society of Tropical Medicine and Hygiene, 51: 5, 1957

The mean filtration pressure of leukocyte suspensions and its relation to the passage of leukocytes through nuclepore filters and capillary networks. Microcirculation 1(4): 237-250, 1994

The detection of microfilariae using the capillary haemotocrit tube method. Tropical Animal Health and Production 3(1): 23-25, 1971

The detection of microfilariae using the capillary hematocrit tube method. Tropical Animal Health and Production 3(1): 23-25, 1971

Modification of nuclepore filtration technique for microfilaria detection. Japanese Journal of Experimental Medicine 50(5): 393-394, 1980

Column separation and membrane filtration: a routine method for the detection of African trypanosomes in human blood. Annales de la Societe Belge de Medecine Tropicale 53(2): 109-112, 1973