+ Translate

Evaluation of a membrane (Nuclepore) filtration method using capillary blood for the detection of microfilariae

, : Evaluation of a membrane (Nuclepore) filtration method using capillary blood for the detection of microfilariae. Annals of Tropical Medicine and Parasitology 76(3): 339-345

The efficacy of a membrane (Nuclepore) filtration technique using [human ] capillary blood (100 mm3) for the detection of blood-circulating microfilariae was investigated during a prevalence survey for Wuchereria bancrofti and Mansonella ozzardi in north Trinidad by comparing results thus obtained with results from conventional thick smear preparations (25 mm3) and from membrane (Nuclepore) filtration of venous blood (1 ml). Prevalence rates of W. bancrofti were 14% by thick smear, 17% by filtration of capillary blood and 16% by filtration of venous blood. For M. ozzardi the corresponding rates were 27, 29 and 31%. A total of 389 persons was examined by all 3 methods. Microfilaria detection rates for both of the filtration techniques were significantly higher than by thick smear preparation, with the exception of results for M. ozzardi obtained by filtration of capillary blood. There was no significant difference between the number of positive persons detected by filtration of capillary blood and filtration of venous blood. Significantly higher microfilaria densities/unit volume of blood were recorded by thick smear preparation and filtration of capillary blood than by filtration of venous blood; results for the 2 capillary blood techniques were similar. In the detection of low density microfilaremias, membrane filtration of capillary blood provides a practical and sensitive alternative to venous blood filtration and has the additional advantage of capillary blood collection rather than venepuncture.

(PDF 0-2 workdays service)

Accession: 005395213

PMID: 6751251

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Nathan, M.; Lambourne, A.M.nteil, S., 1982: Evaluation of a membrane (Nuclepore) filtation method using capillary blood for the detection of microfilariae. Wuchereria bancrofti, Mansonella ozzardi, human, prevalence, comparison of microfilariae detection rates and microfilaria counts by 3 methods (membrane (Nuclepore) filtration of capillary blood, membrane filtration of venous blood, and conventiona...

McMahon, J.E.; Marshall, T.F.; Vaughan, J.P.; Abaru, D.E., 1979: Bancroftian filariasis: a comparison of microfilariae counting techniques using counting chamber, standard slide and membrane (nuclepore) filtration. Three techniques were compared for counting microfilariae of Wuchereria bancrofti in [human] blood specimens: a standard slide method, the counting chamber technique (CCT) and a membrane filtration technique (MFCT). The CCT was much more sensitive...

Abaru, D.E.; Denham, D.A., 1976: A comparison of the efficiency of the Nuclepore and Millipore filtration systems for detecting microfilariae. Using Brugia pahangi or Dirofilaria immitis microfilariae as the test organisms no significant difference in efficiency could be detected between nuclepore and millipore filters. It was found that 0.7% of microfilariae passed through the millipore...

Partono, F.; Cross, J.H.; Purnomo; Demijati, S., 1973: Evaluation of thick smear, Knott and membrane filtration methods for demonstrating microfilariae in blood. An evaluation was made on the detection of Wuchereria bancrofti microfilariae in a 20 mm3 thick blood smear, one ml aliquots of venous blood examined by the Knott technique and one ml of venous blood examined by membrane filtration. 105 people wer...

Webber, W.A.F., 1957: An improved technique for the filtration of microfilariae from venous blood, and a method of recovering the microfilariae alive. Transactions of the Royal Society of Tropical Medicine and Hygiene, 51: 5

Eppihimer, M.J.; Lipowsky, H.H., 1994: The mean filtration pressure of leukocyte suspensions and its relation to the passage of leukocytes through nuclepore filters and capillary networks. To quantitatively evaluate the deformability of bulk suspensions of leukocytes (WBCs), account for the variance of individual cell mechanical properties, and deduce the potential for WBC entrapment within the capillary network in response to alter...

Collins, J.D., 1971: The detection of microfilariae using the capillary haemotocrit tube method. Tropical Animal Health and Production 3(1): 23-25

Collins, J.D., 1971: The detection of microfilariae using the capillary hematocrit tube method. Tropical Animal Health and Production 3(1): 23-25

Tanaka, H.; Shibuya, T., 1980: Modification of nuclepore filtration technique for microfilaria detection. The nuclepore filtration technique was modified by filtering the citrated blood sample through the nuclepore membrane before haemolysis and later washing the membrane holder with 0.1% sodium bicarbonate solution.

Van Meirvenne, N.; Janssens, P.G.; Magnus, E.; Moors, A., 1973: Column separation and membrane filtration: a routine method for the detection of African trypanosomes in human blood. Annales de la Societe Belge de Medecine Tropicale 53(2): 109-112