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Evaluation of a monoclonal antibody based enzyme immunoassay abbott pap eia kit for prostatic acid phosphatase


, : Evaluation of a monoclonal antibody based enzyme immunoassay abbott pap eia kit for prostatic acid phosphatase. Nishinihon Journal of Urology 46(4): 801-807

The clinical application of enzyme immunoassay (EIA) for prostatic acid phosphatase (PAP) is reported. PAP concentration was measured by ABBOTT PAP-EIA kit. Serum samples were collected from 56 healthy individuals (30 males, 26 females), and 35 patients with benign prostatic hypertrophy, 10 patients with chronic prostatitis, 20 patients with chronic renal failure, 16 patients (83 samples) with prostatic cancer, and 18 patients with various other malignancies (renal cell carcinoma (2), renal pelvic tumor (1), ureter tumor (1), bladder tumor (4), testicular cancer (3), lung cancer (2) cases and gastrointestinal cancer 5 cases.) Intraassay coefficients of variation (% CV) arranged from 0.39-8.82; interassay % CV ranged from 6.63 to 12.4. The recovery rates in the recovery test were 100.8 .+-. 10.5%. PAP concentrations in normal males (n = 30) were 0.924 .+-. 0.35 ng/ml, and normal females (n = 22) were 0.821 .+-. 0.44 ng/ml. Upper limit of normal-ranged PAP concentration was 1.624 ng/ml (mean + 2 SD). Significant correlation (r = 0.95. P < 0.01) was seen between RIA [radioimmunoassay] and EIA method. PAP levels in benign prostatic hypertrophy (n = 35) were 0.787 .+-. 0.32 ng/ml. False positive results were not observed. Elevations in PAP concentrations above 1.624 ng/ml were observed in 25% of patients with stage A prostatic cancer, 25% with stage B, 100% with stage C and 100% with stage D prior to treatment. PAP levels measured by ABBOTT PAP-EIA kit were as useful for the diagnosis of prostatic cancer as RIA method.

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Related references

Koshida, K.; Naito, K.; Hisazumi, H., 1987: Evaluation of a monoclonal antibody-based enzyme immunoassay (IQ(Bio) PAP-AELIA kit) for prostatic acid phosphatase. The clinical application of enzyme immunoassay (EIA) for prostatic acid phosphatase (PAP) is reported. PAP concentration was measured by an IQ(Bio)PAP-AELIA kit. Serum samples were collected from 20 healthy individuals, 31 patients with benign pro...

Wirth, M.P.; Gomes de Oliveira, J.; Frohmüller, H.G., 1984: Monoclonal antibody-based radioimmunoassay compared with conventional enzyme immunoassay in the detection of prostatic acid phosphatase. Determination of serum prostatic acid phosphatase by a monoclonal antibody-based radioimmunoassay (RIA) was compared to a polyclonal antibody-based enzyme immunoassay (EIA) to study the clinical value of both test systems. The 97.5 percentile of 4...

Oosterom R.; Leijnse B.; Bogdanowicz J.; Schroeder F.H., 1986: Evaluation of an enzyme immunoassay for prostatic acid phosphatase utilizing monoclonal antibodies. World Journal of Urology 4(3): 153-158

Davies, S.N.; Gochman, N., 1983: Evaluation of a monoclonal antibody-based immunoradiometric assay for prostatic acid phosphatase. This report evaluates a new immunoradiometric assay for prostatic acid phosphatase in serum, based on a dual monoclonal antibody reaction system (Hybritech-TANDEM). A solidphase antibody binds the acid phosphatase molecule and a second monoclonal...

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Taylor, E.H.; Gadsden, R.H., 1984: Serum prostatic acid phosphatase (PAP): monoclonal enzyme-linked immunoassay compared to polyclonal radioimmunoassay. A new enzyme linked immunosorbent assay (ELISA) kit which utilizes a monoclonal antibody against prostatic acid phosphatase (PAP) was compared with current radioimmunoassay (RIA) methodology which uses a polyclonal antibody. Both assays are double...

Kuo, C.Y.; Chen, K.W.; Fu, J.; Lam, K.W.; Lee, C.Y., 1989: Generation of monoclonal antibodies to prostatic acid phosphatase isoenzyme 2 and application in solid-phase enzyme immunoassay. Monoclonal antibodies specific to prostatic acid phosphatase (PAP) isoenzyme 2 were generated by using an improved hybridoma technique. After three subcutaneous immunizations and three intravenous boosters, cell fusion experiments were performed....

Cooper E.H.; Glashan R.; Robinson M.R.G.; Morgan D.B.; Trautner K., 1981: The evaluation of a new enzyme immunoassay for the measurement of prostatic acid phosphatase. An enzyme immunoassay for prostatic acid phosphatase (PAP) was assessed. An upper limit of normal is set at 1.8 .mu.g/l. There is a very low incidence of raised levels in chronic disease or cancers other than those of the prostate. Patients with w...

Moriyama, N.; Taniguchi, J.; Hara, M.; Fukutani, K.; Matsushima, H.; Murakami, T.; Isurugi, K.; Kawabe, K.; Niijima, T., 1987: Immunohistochemical study of prostatic acid phosphatase and prostatic specific antigen in prostatic carcinoma--a comparison of monoclonal antibody to polyclonal antibody. Nihon Hinyokika Gakkai Zasshi. Japanese Journal of Urology 78(11): 1992-1997

Sugiyama, T.; Hiei, T.; Kato, N.; Mitsuya, H.; Noguchi, H.Z.; Senda, K.; Sakaki, T., 1981: The evaluation of a new radio immunoassay kit for prostatic acid phosphatase 1. setting upper limit of serum prostatic acid phosphatase level. A new radioimmunoassay kit (Dab PAP [prostatic acid phosphatase] kit) was evaluated. Results of precision and accuracy tests were satisfactory. In the clinical phase, serum PAP level was measured in the serum obtained from 22 patients with treated...