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Evaluation of a monoclonal antibody based enzyme immunoassay abbott pap eia kit for prostatic acid phosphatase

, : Evaluation of a monoclonal antibody based enzyme immunoassay abbott pap eia kit for prostatic acid phosphatase. Nishinihon Journal of Urology 46(4): 801-807

The clinical application of enzyme immunoassay (EIA) for prostatic acid phosphatase (PAP) is reported. PAP concentration was measured by ABBOTT PAP-EIA kit. Serum samples were collected from 56 healthy individuals (30 males, 26 females), and 35 patients with benign prostatic hypertrophy, 10 patients with chronic prostatitis, 20 patients with chronic renal failure, 16 patients (83 samples) with prostatic cancer, and 18 patients with various other malignancies (renal cell carcinoma (2), renal pelvic tumor (1), ureter tumor (1), bladder tumor (4), testicular cancer (3), lung cancer (2) cases and gastrointestinal cancer 5 cases.) Intraassay coefficients of variation (% CV) arranged from 0.39-8.82; interassay % CV ranged from 6.63 to 12.4. The recovery rates in the recovery test were 100.8 .+-. 10.5%. PAP concentrations in normal males (n = 30) were 0.924 .+-. 0.35 ng/ml, and normal females (n = 22) were 0.821 .+-. 0.44 ng/ml. Upper limit of normal-ranged PAP concentration was 1.624 ng/ml (mean + 2 SD). Significant correlation (r = 0.95. P < 0.01) was seen between RIA [radioimmunoassay] and EIA method. PAP levels in benign prostatic hypertrophy (n = 35) were 0.787 .+-. 0.32 ng/ml. False positive results were not observed. Elevations in PAP concentrations above 1.624 ng/ml were observed in 25% of patients with stage A prostatic cancer, 25% with stage B, 100% with stage C and 100% with stage D prior to treatment. PAP levels measured by ABBOTT PAP-EIA kit were as useful for the diagnosis of prostatic cancer as RIA method.

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