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Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation


, : Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation. Bone Marrow Transplantation 1(3): 297-301

A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.

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Accession: 005395291

PMID: 3332140

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Related references

Ball E.D., 1988: Monoclonal antibody and complement mediated purging of bone marrow for autologous bone marrow transplantation in acute myeloblastic leukemia. Journal of Cellular Biochemistry Supplement (12 PART C): 65

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