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Evaluation of a nephelometric method for determining cereal alpha amylase

Cereal Chemistry 57(6): 411-415
Evaluation of a nephelometric method for determining cereal alpha amylase
A nephelometric assay adapted from a clinical method was evaluated for suitability in determining .alpha.-amylase levels in sprouted soft white wheat. In the manual mode of machine operation, the velocity of decline in light scattering was directly proportional to the amount of enzyme assayed. The accurate limits of the assay performed in the automated mode were defined as 0-720 machine units of activity, with a commercial .beta.-limit dextrin as substrate. This substrate was more reactive than amylopectin and less subject to .beta.-amylase interference. The automated assay yielded data that correlated highly with falling number results.

Accession: 005395311

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