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Evaluation of a nephelometric method for determining cereal alpha amylase

, : Evaluation of a nephelometric method for determining cereal alpha amylase. Cereal Chemistry 57(6): 411-415

A nephelometric assay adapted from a clinical method was evaluated for suitability in determining .alpha.-amylase levels in sprouted soft white wheat. In the manual mode of machine operation, the velocity of decline in light scattering was directly proportional to the amount of enzyme assayed. The accurate limits of the assay performed in the automated mode were defined as 0-720 machine units of activity, with a commercial .beta.-limit dextrin as substrate. This substrate was more reactive than amylopectin and less subject to .beta.-amylase interference. The automated assay yielded data that correlated highly with falling number results.

Accession: 005395311

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Related references

Osborne B.G.; Douglas S.; Fearn T.; Moorhouse C.; Heckley M.J., 1981: Collaborative evaluation of a rapid nephelometric method for the measurement of alpha amylase in flour. An interlaboratory investigation of the Perkin-Elmer model 191 grain amylase analyzer was conducted in the UK. The instrument was calibrated against the Farrand method for bread [wheat] flours on the amylase-2 scale only; the conversion factor was...

Fretzdorff, B.; Weipert, D., 1983: Nephelometric method for the determination of alpha -amylase in cereals. The performance of a Grain Amylase Analyzer (Perkin-Elmer) was examined over 4 months during which >150 samples were analysed. Statistical evaluation of the results for 60 samples each of wheat and rye showed good correlation with results by ot...

Hsu E.; Varriano Marston E., 1983: Comparison of nephelometric and phadebas methods of determining alpha amylase activity in wheat flour supplemented with barley malt. .alpha.-Amylase activity in wheat flour supplemented with different levels of barley malt was determined by nephelometric and Phadebas methods. The methods were highly correlated with each other, indicating that one method can be used to predict t...

Fretzdorff B.; Weipert D., 1983: A nephelometric method for the measurement of alpha amylase ec activity in cereals. The method is based on the principle of nephelometry, i.e., the measurement of light scattered by particles in suspension; the decrease of turbidity of a .beta.-limit dextrin suspension effected by .alpha.-amylase provides a direct measure of enzy...

Fretzdorff, B.W.ipert, D., 1983: A nephelometric method for the measurement of alpha-amylase activity in cereals. Zeitschrift fur Lebensmittel Untersuchung und Forschung77(3): 167-172

Campbell, J.A., 1980: A new method for detection of sprout damaged wheat using a nephelometric determination of alpha-amylase activity. The use of the Perkin-Elmer Model 191 Grain Amylase Analyzer for simple and rapid determinations of alpha -amylase activity in wheat is described. The instrument measures the rate of change of light scattered from the substrate suspension. The mea...

Campbell, J.A., 1980: A collaborative study of alpha-amylase in sprouted wheat determined by the nephelometric methods (grain amylase analyzer). The study revealed significant correlations between amylograph and falling number determinations, on the one hand, and the results of the nephelometric method for the determination of alpha -amylase by light scattering, on the other. The method is...

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