EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a new continuous colorimetric method for determination of serum pseudocholinesterase ec 3.1.1.8 catalytic activity and its application to a centrifugal fast analyzer


Journal of Clinical Chemistry and Clinical Biochemistry 22(10): 671-676
Evaluation of a new continuous colorimetric method for determination of serum pseudocholinesterase ec 3.1.1.8 catalytic activity and its application to a centrifugal fast analyzer
The evaluation of a new commercially available assay system for the determination of serum pseudocholinesterase catalytic activity and its application to a kinetic analyzer was reported. The assay is based on the colorimetric method of Okabe et al. choline, liberated from benzoylcholine by pseudocholinesterase, is oxidized by choline oxidase (EC 1.1.3.17) to betaine with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a colored compound with maximal absorbance at 500 nm. The procedure not only has the advantage of being continuous, colorimetric and totally enzymatic but also appears to be precise (between-day analysis gives coefficient of variation between 3.5 and 5.6%) and accurate: the results obtained from normal and pathological sera [from humans] show excellent correlation with those obtained by the alternative procedures employing propionylthiocholine, acetylthiocholine and butyrylthiocholine as substrates.


Accession: 005395356



Related references

Evaluation of a new continuous colorimetric method for determination of serum pseudocholinesterase catalytic activity and its application to a centrifugal fast analyser. Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift für Klinische Chemie und Klinische Biochemie 22(10): 671-676, 1984

Fast immunochemical determination of proteins with gemsaec centrifugal analyzer application to serum transferrin and immuno globulin a assay. Annales de Biologie Clinique 35(3): 227-236, 1977

Colorimetric determination of serum bile acids with a centrifugal analyzer. Journal of Clinical Chemistry & Clinical Biochemistry 23(9): 589, 1985

Enzymatic determination of acetate in serum or plasma using a centrifugal fast analyzer. Clinica Chimica Acta 121(1): 43-50, 1982

Assessment of colorimetric enzymatic determination of triglyceride, by manual and centrifugal analyzer techniques, and comparison with a CDC standardized method. Clinical Biochemistry 14(1): 28-31, 1981

An improved method for the measurement of inorganic phosphate in serum with a centrifugal fast analyzer. Clinical Chemistry: 961, 1975

Assessment of colorimetric enzymatic determination of tri glyceride by manual and centrifugal analyzer techniques and comparison with a center for disease control standardized method. Clinical Biochemistry 14(1): 28-31, 1981

Application of a sensitive uricase ec 1.7.3.3 peroxidase ec 1.11.1.7 coupled reaction to a centrifugal fast analyzer for the determination of uric acid. Clinica Chimica Acta 116(3): 301-310, 1981

Rapid kinetic, turbidimetric microassay of serum lipase activity using the centrifugal fast analyzer. Zeitschrift für Klinische Chemie und Klinische Biochemie 12(5): 265-265, 1974

Serum alkaline phosphatase: total activity and isoenzyme determinations made by use of the centrifugal fast analyzer. Clinical Chemistry 18(12): 1468-1474, 1972