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Evaluation of a new presumptive medium for group D streptococci


, : Evaluation of a new presumptive medium for group D streptococci. Applied and Environmental Microbiology 33(5): 1149-1155

A new medium designated as D streptococcus-enterococcus broth was formulated and evaluated for the enrichment and isolation of strains of serological group D streptococci [Streptococcus spp.]. This medium was made by modifying Todd-Hewitt broth. Most-probable-number [MPN], multiple-tube and membrane filter techniques were employed to estimate the numbers of enterococci in known cultures, waste-water and other samples. Preliminary MPN counts with this medium were as much as 3 logs higher than those counts obtained from 4 other media with which it was compared. The methodology for using this medium to estimate the numbers of group D streptococci in water is discussed.

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Accession: 005395417

PMID: 406842

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