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Evaluation of a new thin layer chromatographic confirmation technique for positive emit enzyme multiplied immunoassay cannabinoid urine samples


, : Evaluation of a new thin layer chromatographic confirmation technique for positive emit enzyme multiplied immunoassay cannabinoid urine samples. Research Communications in Substances of Abuse 6(1): 1-10

A bonded phase adsorption thin-layer chromatography (BPA-TLC) confirmation procedure was tested for urine samples found positive by the [enzyme multiplied immunoassay] EMIT-dau cannabinoid assay. A total of 803 samples were tested including 47 EMIT-dau negatives. The BPA-TLC system was very effective confirming both EMIT positive and negative samples. The BPA-TLC system separates and identifies the major metabolite of .DELTA.9-tetrahydrocannabinol (THC), 110-nor-.DELTA.9-THC-9-Carboxylic acid (THC). When Fast Blue RR was used as the color reagent 647 samples were correctly identified as positive or negative, of the 662 tests, yielding 97.7% efficiently. With Fast Blue B, 138 confirmations of 141 EMIT positive samples yielded 97.9% efficiency. No false positives were observed and after pooling the data, 785 samples of 803 were correctly identified by the BPA-TLC system (97.8%). The BPA-TLC technique is evidently practical, quick, inexpensive and very reliable for the confirmation of EMIT positive cannabinoid urine samples.

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