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Evaluation of a new thin layer chromatographic confirmation technique for positive emit enzyme multiplied immunoassay cannabinoid urine samples

, : Evaluation of a new thin layer chromatographic confirmation technique for positive emit enzyme multiplied immunoassay cannabinoid urine samples. Research Communications in Substances of Abuse 6(1): 1-10

A bonded phase adsorption thin-layer chromatography (BPA-TLC) confirmation procedure was tested for urine samples found positive by the [enzyme multiplied immunoassay] EMIT-dau cannabinoid assay. A total of 803 samples were tested including 47 EMIT-dau negatives. The BPA-TLC system was very effective confirming both EMIT positive and negative samples. The BPA-TLC system separates and identifies the major metabolite of .DELTA.9-tetrahydrocannabinol (THC), 110-nor-.DELTA.9-THC-9-Carboxylic acid (THC). When Fast Blue RR was used as the color reagent 647 samples were correctly identified as positive or negative, of the 662 tests, yielding 97.7% efficiently. With Fast Blue B, 138 confirmations of 141 EMIT positive samples yielded 97.9% efficiency. No false positives were observed and after pooling the data, 785 samples of 803 were correctly identified by the BPA-TLC system (97.8%). The BPA-TLC technique is evidently practical, quick, inexpensive and very reliable for the confirmation of EMIT positive cannabinoid urine samples.

Accession: 005395450

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Related references

Von Meyer L., 1985: Detection of cannabinoids in blood and urine by emit enzyme multiplied immunoassay technique confirmation by thin layer chromatography. The EMIT [enzyme-multiplied immunoassay] technique cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in the urine remain detectable up to 1 wk....

Kogan, M.J.; A.R.zi, J.; Pierson, D.J.; Willson, N.J., 1986: Confirmation of Syva enzyme multiple immunoassay technique (EMIT) d.a.u. and Roche Abuscreen radioimmunoassay (RIA) (125I) urine cannabinoid immunoassays by gas chromatographic/mass spectrometric (GC/MS) and bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) methods. Thirty human urines screened positive by the Syva enzyme multiple immunoassay technique (EMIT) d.a.u. urine cannabinoid assay were also positive for the major marijuana urinary metabolite 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-...

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