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Evaluation of a nondestructive acetylene reduction assay of nitrogen fixation for pasture legumes grown in pots


, : Evaluation of a nondestructive acetylene reduction assay of nitrogen fixation for pasture legumes grown in pots. New Zealand Journal of Experimental Agriculture 6(1): 65-68

Plants of Trifolium repens L. 'Grasslands Huia' (white clover), T. pratense L. 'Grasslands Turoa' (Montgomery red clover), T. hybridum L. (alsike clover), T. pratense L. 'Grasslands Pawera' (broad red clover), T. subterraneum L. 'Tallarook' (subterranean clover), Medicago sativa L. 'Grasslands Wairau' (lucerne) and Lotus pedunculatus Cav. (synonym: L. uliginosus Schkuhr, L. major Sm) 'Grasslands Maku' (lotus) were grown in pots with adequate moisture at day/night temperatures of 15.degree. C/10.degree. C and 23.degree. C/10.degree. C, and under moisture stress at 23.degree. C/10.degree. C. At weekly intervals during 5 and 6 wk growth periods successive non-destructive acetylene reduction (AR) assays were performed by incubating potted plants in 5 liter plastic containers for 2 h with 200 ml acetylene. N2 fixation over the same periods was calculated from chemical analysis of plant tissue. AR and N2 fixation rates were closely related within species and treatments, but the molar ratio of C2H2 reduced: N2 fixed differed between clovers (mean ratio 2.8:1), lucerne (3.4:1) and lotus (4.3:1). Moisture stress increased the AR:N2 fixed ratio, but temperature had no consistent effect. Repeated assays had no effect on herbage DM [dry matter] accumulation and herbage percent N.

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Accession: 005395463

DOI: 10.1080/03015521.1978.10426017

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