EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a preparative method for x ray micro analysis of soft tissue


, : Evaluation of a preparative method for x ray micro analysis of soft tissue. Cytobios 28(109): 17-34

The cryopreparative methods designed for the radioautography of diffusible substances were adapted to produce freeze-fried sections of soft tissues for electron probe microanalysis. The evaluation of these methods for use with X-ray microanalysis with particular reference to: the preservation of cellular morphology, the introduction of structural artefact by ice crystal formation, the preservation of natural elemental distributions under experimental conditions where known quantities of diffusible elements are present, the effects of ice crystal formation upon possible artefactual elemental redistributions, the effect of section thickness on elemental quantitation and the effect of tissue excision on element translocation reported. Freeze-dried sections were prepared from mouse pancreas and from 20% (wt/vol) solutions of bovine serum albumin (BSA) and gelatin containing known amounts of inorganic salts and were analyzed in a scanning electron microscope fitted with energy dispersive X-ray detecting equipment. With the preparative methods used, the morphology of pancreatic acinar cells was well preserved. Acinar cell boundaries, nuclear boundaries, chromatin, nucleoli, ergastoplasm and zymogen granules were readily discernible. Ice crystals were present within sections of BSA, but 70% had a cut surface area < 1 .mu.m2 and over 90% had a cut surface area of < 2 .mu.m2. Characteristic peak-to-continuum ratios of elements in the BSA sections remained constant over an order of magnitude change in magnification. Elemental redistributions were not detected until the magnification was such that the analyzed area fell totally within the confines of a single ice crystal. No differences in elemental peak-to-continuum values were obtained between 2 and 4 .mu.m sections of the gelatin-salt solution. Tissue excision did not cause element translocations when compared to tissues frozen in situ. Apparently this method is valid for preparing tissues for microanalysis under these conditions (analysis of nuclear, cytoplasmic and secretory compartments) and is limiting only when analyses are conducted at very high magnifications.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005395521

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Neymark L. A.; Mirkina S. L.; Rublev A. G.; Rizvanova N. G.; Kuzina N. A.; Rudakova Zh N., 1987: Isotope age of the granites in the Irel'skiy Complex, Baikal region. Izvestiya Akademii Nauk SSSR Seriya Geologicheskaya

Lumley, Mark A.; Asselin, Linda A.; Norman, Sally, 1997: Alexithymia in chronic pain patients. Prior studies of alexithymia in chronic pain patients have used unvalidated alexithymia measures or have not controlled for treatment-seeking status. In this study, we compared 30 patients with chronic pain and patients seeking treatment for two o...

Lefaucheur, J. , P.; Brugieres, P.; Menard-Lefaucheur, I.; Wendling, S.; Pommier, M.; Bellivier, F., 2007: Un cas illustratif de l'interet de la SMTr guidee par neuronavigation dans le traitement de la depression. Repetitive transcranial magnetic stimulation (rTMS) of the prefrontal cortex has been thought to have great potential to treat refractory depression since the first studies published ten years ago. However, one of the potential limitations of rTM...

Dyuzhikov O. A., 1990: Norilsk nickel-bearing region of the Yenisei-Olenek ore belt. Pages 84-91 1990

El Hinnawi, E., 1981: Energy for rural development. A time to act. The article describes the UNEP projects for improving energy supply in rural areas, using wind, water and solar energy as well as biogas.

Cui Yanshan; Wang Qingren, 2003: Effect of sulfur fertilization on yield production and sulfur content of crops and pasture in north China. Pot experiment on 3 crops (wheat, corn, oilseed rape) and a pasture (clover) under 5 levels (0, 5, 10, 20, 40 mgcntdotkg-1) of sulfur (S) was carried out to study S application on yield production, S contents and their relationship of these crops....

Abo Kaied, H. M. H.; El Sweify, A. H. H., 2003: Simultaneous selection for fiber yield and stability of crop performance in jute. An experiment was conducted during 2000-01 in Egypt on Corchorus capsularus [C. capsularis] cultivars UPC 94, UPC 7716, IRC 212, IRC 321, JRC 7447, DC 9105, and PADMA and C. olitoris cv. S1 to evaluate yield potential via yield stability statistic...

Lee, W. C.; Jeng, L. B. B.; Wang, C. C.; Chen, M. F.; Chien, R. N.; Chiu, C. T.; Ling, D. Y.; Liaw, Y. F.; Chang, C. H., 1996: Long-term immunoprophylaxis for hepatitis B virus reinfection after liver transplantation. Transplantation Proceedings 28(3): 1699-1700

Goldstein L.J.; Rypins E.B., 1989: Nonlinear spice models for physiologic systems. Most biological systems are nonlinear but investigators interested in modeling them often make linear approximations to avoid performing tedious manual calculations. SPICE (Simulation Program with Integrated Circuit Emphasis, developed at the Univ...

Silba, J., 1983: A new species of Decussocarpus De Laub. (Podocarpaceae) from Brazil. Phytologia 54(6): 460-462