EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a proprietary broth medium for microdilution susceptibility testing of nutritionally fastidious bacteria






Journal of Clinical Microbiology 24(5): 701-704

Evaluation of a proprietary broth medium for microdilution susceptibility testing of nutritionally fastidious bacteria

For microdilution susceptibility tests with nutritionally fastidious microorganisms, a new clear broth medium developed at Micro-Media Systems, Inc., Potomac, Md., was evaluated in a three-laboratory collaborative study. Replicate tests were performed with 80 isolates (51 Streptococcus spp., 27 Haemophilus influenzae isolates, and 2 Neisseria meningitidis isolates) against 15 antimicrobial agents. In standard 100-microliters volumes, results of tests in the new broth medium were comparable to those in the reference medium (Mueller-Hinton broth with 2 to 3% lysed horse blood), but MICs were somewhat easier to read in the new broth medium. Results of similar tests in smaller panels, containing 40 microliters in each well, were less satisfactory; i.e., growth failures and poorly defined endpoints were more commonly encountered. With drugs other than erythromycin or clindamycin, the 40-microliters panels provided MICs which compared favorably with those obtained by standard reference methods.


Accession: 005395540

PMID: 3095368



Related references

Cohen, S.; Carroll, K., 1997: Comparison of the E-test to broth microdilution for susceptibility testing of viridans and nutritionally variant streptococci. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 207

Carroll, K.C.; Cohen, S.; Newcomb Gayman, P.; Mottice, S., 1994: Comparison of the E-test, FOX fastidious panel with a reference broth microdilution method for susceptibility testing of outbreak strains of Neisseria meningitidis. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 34(0): 121

Jorgensen, J.H.; Doern, G.V.; Ferraro, M.J.; Knapp, C.C.; Swenson, J.M.; Washington, J.A., 1992: Multicenter evaluation of the use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae and development of quality control limits. A five-laboratory collaborative study was undertaken to determine the precision and accuracy of broth microdilution susceptibility tests of Streptococcus pneumoniae isolates peformed with Haemophilus test medium (HTM) compared with tests performed...

Cotton J.L.; Barry A.L.; Packer R.R.; Jones R.N., 1986: Three laboratory evaluation of the micro media systems fastidious microdilution susceptibility test medium. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 380

Jorgensen J.H.; Maher L.A.; Howell A.W., 1989: Use of haemophilus test medium htm for broth microdilution susceptibility testing of streptococcus pneumoniae. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 405

Martinez Martinez, L.; Ortega, M.C.; Suarez, A., 1994: Comparison of the E-test with broth microdilution for susceptibility testing of coryneform bacteria. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 34(0): 121

Jorgensen, J.H.; Maher, L.A.; Howell, A.W., 1990: Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae. A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolate...

Wallmann, Jürgen.; Böttner, A.; Goossens, L.; Hafez, H.Mohamed.; Hartmann, K.; Kaspar, H.; Kehrenberg, C.; Kietzmann, M.; Klarmann, D.; Klein, Günter.; Krabisch, P.; Kühn, T.; Luhofer, G.; Richter, A.; Schulz, B.; Schwarz, S.; Sigge, C.; Traeder, W.; Waldmann, K-Heinz.; Werckenthin, C.; Zschiesche, E., 2006: Results of an interlaboratory test on antimicrobial susceptibility testing of bacteria from animals by broth microdilution. A standard operating procedure for the determination of minimum inhibitory concentrations (MICs) of antimicrobial agents by the broth microdilution method was developed and evaluated for its fitness for use in an interlaboratory ring trial involvi...

Martínez-Martínez, L.; Ortega, M.C.; Suárez, A.I., 1995: Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria. The susceptibilities of 135 coryneform bacteria isolated from clinical samples to ampicillin (AMP), cephalothin (CR), cefoxitin (FOX), cefotaxime (CTX), erythromycin (E), ciprofloxacin (CIP), tetracycline (TE), amikacin (AK), vancomycin (VA), and...

Koehling, H.Luise.; Willinger, B.; Buer, J.; Rath, P-Michael.; Steinmann, J., 2015: Comparative evaluation of a new commercial colorimetric microdilution assay (SensiQuattro Candida EU) with MIC test strip and EUCAST broth microdilution methods for susceptibility testing of invasive Candida isolates. Candidemia is an important cause of morbidity and mortality in immunosuppressed patients. Candida isolates must be cultivated, identified, and tested for susceptibility. We compared the performance of a new colorimetric broth microdilution panel (...