+ Translate

Evaluation of a quantitative immunoturbidimetric assay for rheumatoid factors

, : Evaluation of a quantitative immunoturbidimetric assay for rheumatoid factors. Clinical Chemistry 32(10): 1890-1894

A quantitative immunoturbidimetric assay for rheumatoid factors (RF) is described, based on the immunoprecipitation between aggregated human IgG and rheumatoid factors in serum. The resulting turbidity is measured photometrically at 340 nm. The method is standardized against the WHO international reference preparation and the results are expressed in international units per milliliter (int. units/mL). Results correlate well with those by different latex-agglutination techniques (r = 0.80-0.96). The correlation with Waaler-Rose test modifications were 0.75 and 0.92. The within-run and between-run coefficients of variations were respectively from 1.2 to 2.6% and 1.3 to 1.9% for high and low RF concentrations. Quantitative and reproducible results, together with high throughout of samples and compatibility with most clinical chemistry analyzers and photometers, make this new assay well suited for routine screening and monitoring of rheumatoid factors.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005395563

PMID: 3530533

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Mazurenko L. L.; Soloviev V. A. (Soloviyev V. A); Belenkaya I.; Ivanov M. K.; Pinheiro L. M., 2002: Mud volcano gas hydrates in the Gulf of Cadiz. Terra Nova 14(5): 321-329

Adelseck C. G. Jr.; Roth P. H., 1975: Test morphology and crystal size; controlling factors in the selective dissolution of Coccolithophorida and foraminifera species. Abstracts with Programs - Geological Society of America 7(7): 969-970

Kraetke, O.; Wiesner, B.; Eichhorst, J.; Furkert, J.; Bienert, M.; Beyermann, M., 2006: Dimerization of corticotropin-releasing factor receptor type 1 is not coupled to ligand binding. As described previously, receptor dimerization of G protein-coupled receptors may influence signaling, trafficking, and regulation in vivo. Up to now, most studies aiming at the possible role of receptor dimerization in receptor activation and sig...

Kornfeld Joseph A., 1971: Interest is high in new East Colorado oil play. World Oil 172(2): 55

Levine, D.N.; Lee, J.M.; Fisher, C.M., 1993: The visual variant of Alzheimer's disease: a clinicopathologic case study. A 59-year-old man developed problems with reading and driving. When first examined, he had great difficulty locating and identifying items by sight. Visual acuity was normal, but contrast sensitivity for low spatial frequencies was severely impair...

Stein, A; Riley, J; Halberg, N., 2001: Issues of scale for environmental indicators. The value of environmental indicators largely depends upon the spatial and temporal scale that they represent. Environmental indicators are dependent upon data availability and also upon the scale for which statements are required. As these may no...

IIda H.; Rhodes C.G.; Yamamoto Y.; Jones T.; De Silva R.; Araujo L.I., 1990: Quantitative measurement of myocardial metabolic rate of oxygen in man using oxygen 15 and positron emission tomography. Circulation 82(4 SUPPL 3): III614

Saha, A. K.; Deshpande, M. V.; Kapadnis, B. P., 2001: Studies on survival of Rhizobium in the carriers at different temperatures using green fluorescent protein marker. The present work was undertaken to use green fluorescent protein marker to monitor population of Rhizobium transformant in low-cost carriers in comparison with lignite. The viability of the Rhizobium transformant carrying the reporter gene gfp, wa...

Yagi M.; Shibukawa A.; Nakagawa T., 1990: Direct injection analysis of ketoprofen enantiomers in plasma using column switching high performance liquid chromatography system. A high-performance liquid chromatography system was developed for the stereoselective determination of ketoprofen enantiomers in human plasma following direct sample injection. The system comprised of a pretreatment column and a chiral separation...

Aldea Munoz, S., 2002: Sublimation and happiness in the current world. The principal objective of this project is that or analyzing the Freudian concepts of sublimation and of happiness, adapting them to our era. Civilization, according to Freud, is constructed on the basis of the repression and sublimation of our ba...