EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a quantitative immunoturbidimetric assay for rheumatoid factors


Clinical Chemistry 32(10): 1890-1894
Evaluation of a quantitative immunoturbidimetric assay for rheumatoid factors
A quantitative immunoturbidimetric assay for rheumatoid factors (RF) is described, based on the immunoprecipitation between aggregated human IgG and rheumatoid factors in serum. The resulting turbidity is measured photometrically at 340 nm. The method is standardized against the WHO international reference preparation and the results are expressed in international units per milliliter (int. units/mL). Results correlate well with those by different latex-agglutination techniques (r = 0.80-0.96). The correlation with Waaler-Rose test modifications were 0.75 and 0.92. The within-run and between-run coefficients of variations were respectively from 1.2 to 2.6% and 1.3 to 1.9% for high and low RF concentrations. Quantitative and reproducible results, together with high throughout of samples and compatibility with most clinical chemistry analyzers and photometers, make this new assay well suited for routine screening and monitoring of rheumatoid factors.


Accession: 005395563

PMID: 3530533



Related references

Evaluation of iron status in anemic patients with rheumatoid arthritis using an automated immunoturbidimetric assay for transferrin receptor. Clinical Chemistry and Laboratory Medicine 38(12): 1297-1300, 2001

Quantitative determination of fibronectin (FN): evaluation of immunoturbidimetric and rate nephelometric techniques for FN assay in the different biologic materials. Allergologia et Immunopathologia 23(6): 285-289, 1995

Evaluation of the UNI-KIT RF, UNI-KIT ASO and UNI-KIT CRP three immunoturbidimetric assays for the measurement of rheumatoid factors anti-streptolysin O and C-reactive protein in human serum on COBAS instruments. Clinical Chemistry 39(6): 1253, 1993

Measuring rheumatoid factor in nonrheumatoid subjects: immunoturbidimetric assay, latex slide test, and enzyme-linked immunosorbent assay compared. Clinical Chemistry 37(10 Pt 1): 1766-1769, 1991

Fully quantitative and automated assay for rheumatoid factors and its diagnostic value for rheumatoid arthritis. British Journal of Rheumatology 27(ABSTR SUPPL 2): 29, 1988

Immunoturbidimetric assay for rheumatoid factor using an enzyme immunoassay microplate reader. Journal of Clinical Pathology 41(2): 228-230, 1988

Measurement of lipoprotein using a quantitative immunoturbidimetric assay. Clinical Chemistry & Laboratory Medicine 37(SPEC SUPPL ): S268, June, 1999

Quantitative determination of microalbuminuria by an automated immunoturbidimetric assay. Clinical Chemistry 36(6): 997, 1990

Quantitative determination of apolipoprotein a i and b with a rapid immunoturbidimetric assay. Clinical Chemistry 33(6): 897-898, 1987

Quantitative of human apolipoprotein B100 by immunoturbidimetric assay. Journal of West China University of Medical Sciences 28(1): 109-112, 1997