EurekaMag.com logo
+ Translate

Evaluation of a radio immunoassay for immuno globulin m class antibodies against cytomegalovirus


, : Evaluation of a radio immunoassay for immuno globulin m class antibodies against cytomegalovirus. British Journal of Experimental Pathology 61(5): 512-520

A solid-phase radioimmunoassay (RIA-IgM) test for cytomegalovirus (CMV)-specific IgM antibodies is described and its potential diagnostic usefulness in adult patients is evaluated. IgM antibodies were readily detected by RIA in early convalescent sera from patients with serologically confirmed primary CMV infection, and in sera from patients with CMV mononucleosis. Serum specimens obtained several months after primary infection gave either negative results or low antibody titers, indicating that the IgM response is transient in these patients. CMV-specific IgM was also detected in 7 of 10 patients with symptoms and supportive serological evidence suggestive of recent active CMV infection. Conversely, 12 patients who were thought not to have experienced recent primary CMV infection, but whose sera gave high complement-fixing (CF) antibody titers, and 14 of 15 renal transplant recipients with reactivated CMV gave negative results in the RIA-IgM test. Detectable IgM antibody production is probably a constant feature in patients with primary infection but not in patients who experience mild secondary attacks. The RIA-IgM test is highly virus-specific and can be reliably performed with unfractionated serum, provided that false reactivity due to rheumatoid factor is excluded. Thus, RIA is a simple and specific technique which could be usefully applied as a diagnostic tool in a clinical situation and in research.

(PDF 0-2 workdays service)

Accession: 005395569

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Torfason E.G.; Kaellander C.; Halonen P., 1981: Solid phase radio immunoassay of serum immuno globulin g immuno globulin m and immuno globulin a antibodies to cytomegalovirus. A radioimmunoassay (RIA) using polystyrene beads as the solid phase for cytomegalovirus (CMV) antigen and iodinated immunosorbent purified anti-human IgG, IgM and IgA as indicator antibodies was developed for the detection of Ig class-specific ant...

Meurman, O.H.; Ziola, B.R., 1978: Immuno globulin m class rheumatoid factor interference in the solid phase radio immunoassay of rubella specific immuno globulin m antibodies. The interference of Ig[immunoglobulin]M-class rheumatoid factor (RF) in the solid-phase radioimmunoassay (RIA) of rubella virus IgM antibodies was studied. Acute rubella infections did not significantly activate RF. False-positive rubella antibody...

Sarov I.; Levy E.; Aymard M.; Chardonnet Y.; Bosshard S.; Revillard J.P.; Friedman M.; Nord E.; Greiff M.; Haikin H., 1982: Detection of virus specific immuno globulin a antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzyme immunoassay and radio immunoassay techniques. The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in 8 renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-linked...

Roggendorf M.; Zachoval R.; Zoulek G.; Deinhardt F., 1982: Comparison of different evaluation systems for determination of viral antibodies of the immuno globulin g and immuno globulin m class in the enzyme immunoassay. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene 1 Abt Originale A 251(4): 418

Kaye H.S., 1972: Immuno globulin class of influenza antibodies investigated by radio immunoassay. Journal Of Immunology: 849-856

Terho P.; Meurman O., 1981: Chlamydial serum immuno globulin g immuno globulin a and local immuno globulin a antibodies in patients with genital tract infections measured by solid phase radio immunoassay. A solid-phase radioimmunoassay (RIA) for IgG and IgA class antibodies to Chlamydia trachomatis was developed with C. trachomatis serotype L2 as antigen. The assay was sensitive, reproducible and correlated well with an immunofluorescence test (r =...

Sarov I.; Levy E.; Aymard M.; Chardonnet Y.; Bosshard S.; Revillard J.P.; Friedman M.; Nord E.; Greiff M.; Haikin H., 1982: Detection of virus specific immuno globulin a antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzyme linked immuno sorbent assay and radio immunoassay. Israel Journal of Medical Sciences 18(5): 4

Brunner, H.; Schaeg, W.; Brueck, U.; Schummer, U.; Sziegoleit, D.; Schiefer, H.G., 1978: Determination of immuno globulin g immuno globulin m and immuno globulin a antibodies to mycoplasma pneumoniae by an indirect staphylococcal radio immunoassay. An indirect staphylococcal radioimmunoassay (SRIA) was developed for determination of M. pneumoniae antibodies. This test allows the detection of antibodies in various immunoglobulin (Ig) classes similar to the previously described radioimmunoprec...

Sakamoto Y.; Kabe J., 1982: Solid phase radio immunoassay for measurement of immuno globulin g immuno globulin a and immuno globulin m antibodies against aspergillus fumigatus. Five patients with respiratory diseases due to A. fumigatus were studied for measurement of IgG, IgA and IgM antibodies specific for A. fumigatus by solid-phase radioimmunoassay. For both crude and polyssacharide antigens, antibodies of each Ig cl...

Sprouse R.F.; Caldwell C.W.; Everett E.D., 1981: Radio immunoassays that use iodine 125 labeled protein a for determination of histoplasma capsulatum specific immuno globulin g immuno globulin a and immuno globulin m class antibodies in histoplasmosis. A radioimmunoassay for determination of human IgG, IgA and IgM antibodies to H. capsulatum is reported. Yeast-phase antigen from H. capsulatum was adsorbed onto polyvinyl chloride microtiter plates as the solid phase. Human antibodies were allowed...