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Evaluation of a radio immunoassay for plasma acth


, : Evaluation of a radio immunoassay for plasma acth. Journal of Endocrinology 78(3): 309-320

A radioimmunoassay for plasma ACTH was described and evaluated. Rabbit antiserum produced by immunization with [Asp25,Ala26,Gly27,]-.alpha.h-corticotropin-(1-28)-octacosa-peptide (a sequence analog of .**GRAPHIC**. bovine .gamma.-globulin conjugate was used. The antiserum is specific for the NH2-terminal portion of the ACTH molecule and cross-reactivity of human, porcine and rat ACTH in the system was demonstrated. Reasonable agreement was found between estimates obtained by bioassay and radioimmunoassay of the ACTH content of rat pituitary gland incubation media, indicating a close relationship between the sequence of ACTH recognized by the antibodies and the sequence possessing the steroidogenic activity. Measurement of the amount of ACTH in the plasma required the preliminary extraction and concentration of the hormone. Over a range of concentrations, 3.5-3600 pg/ml, extraction recovery was independent of the initial concentration of ACTH in the plasma. Extraction gave rise to no changes in the immunological properties of standard ACTH. The concentration of immunoreactive ACTH in rat plasma was 48 .+-. 36 (SEM [standard error of the mean]) pg/ml in the morning and 106 .+-. 99 pg/ml in the afternoon. Exposure to ether for 5 min and subsequent laparotomy gave rise to a significant increase in the concentration of immunoreactive ACTH in the plasma. The resting level of ACTH and the ACTH response to stress were both significantly higher 1 and 7 days after adrenalectomy. Injection [i.v.] of a hypothalamic extract elicited a considerable rise in the concentration of immunoreactive ACTH in the plasma, but no response was seen after oral administration of this partially purified extract. The sensitivity, precision and specificity of this ACTH radioimmunoassay make it a useful tool for studying pituitary-adrenal physiology.

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Minkov A.; Stanchev A., 1969: Investigation of the zinc content in the sera of patients who have suffered from infectious hepatitis. Revue Internationale d'Hepatologie 19(1): 83-84

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Tsujita, Y.; Endo, A., 1976: Purification and characterization of the 2 molecular forms of aspergillus oryzae acid protease ec 3.4.23.6. The isolation and partial characterization of the acid proteases A1 and A2 (EC 3.4.23.6) from A. oryzae grown on solid bran culture and described. The purified preparations were essentially homogeneous by several criteria including sedimentation a...

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Berendt O., 1974: Effect of the acaricide dinobuton on population density of the predator phytoseiulus persimilis and its prey tetranychus urticae acarina phytoseiidae and tetranychidae. Tidsskrift for Planteavl 78(1): 103-115

Tonini, N. S.; Tomazzoni, M. I.; Maraschin, M. S.; Schneider, J. F., 2002: Identification and analysis of biological and social risk factors of the children born in Cascavel-Parana. An retrospective and descriptive study identifying the risk factors of children born in Cascavel, state of Parana, Brazil from 1996 to 1998 is presented. It is noted that the population in this study is more prone to diseases because of low educat...

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