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Evaluation of a radio immunoassay technique measuring calcitonin in the bovine ovine and porcine species

Acta Veterinaria Scandinavica 21(2): 155-170
Evaluation of a radio immunoassay technique measuring calcitonin in the bovine ovine and porcine species
A heterologous radioimmunoassay system was set up for measuring bovine, ovine and porcine calcitonin (CT). The system consisted of porcine CT used as standard and for the preparation of an iodinated tracer. The antiserum used was raised against ovine CT. For each analysis 25-200 .mu.l blood plasma was used. Practical detection limit was 0.25 .mu.g CT/l of blood plasma. The parallelism between the dose response curves for the porcine CT standard and for the assay of increasing amounts of bovine, ovine and porcine blood plasma showed the suitability of the present assay system to study CT secretion in these species. The reliability of the method was verified by a clearly recognized CT response to Ca infusion.

Accession: 005395574

PMID: 7395679

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