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Evaluation of a rapid bacteriuria screening instrument


, : Evaluation of a rapid bacteriuria screening instrument. Diagnostic Microbiology & Infectious Disease 2(1): 11-16

A newly modified, semiautomated instrument (bacteriuria detection device (BDD)) designed to detect the presence of bacteriuria in < 3 min was compared to quantitative urine culture plating techniques. The instrument consists of a self-enclosed vacuum-filtration-staining system in which a 1 ml urine filtrate is stained on a filter. The resulting color determines the quantitation. Of 525 clinical urine specimens tested, 66 (12.6%) were uninterpretable due to pigment deposition or inability to complete the filtering process (clogging of the filter). Of the remaining 459 specimens, 93 (20.3%) had a plate quantitation colony count of .gtoreq. 105 colony-forming units (CFU)/ml. The BDD detected 94.2% of these positive specimens if only significant pathogens were included (85% if specimens that were probably contaminated were also included). For specimens containing significant pathogens at 104-105 CFU/ml, the BDD detection rate was 41%. The device detected most (94.3%) gram-negative bacilli and enterococci at colony counts of .gtoreq. 105 CFU/ml. The BDD accurately detected 95.6% of specimens with no growth or < 104 CFU/ml. With several proposed modifications, these results suggest that this instrument is potentially useful as a urine screening device in a select population.

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Accession: 005395585

PMID: 6399478

DOI: 10.1016/0732-8893(84)90018-X

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