EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a rapid radiometric method for drug susceptibility testing of mycobacterium tuberculosis






Journal of Clinical Microbiology 13(5): 908-912

Evaluation of a rapid radiometric method for drug susceptibility testing of mycobacterium tuberculosis

A total of 106 isolates of M. tuberculosis were tested for drug susceptibility by the conventional 7H11 plate method and by a new rapid radiometric method using special 7H12 liquid medium with 14C-labeled substrate. Results obtained by the 2 methods were compared for rapidity, sensitivity and specificity of the new test method. There was 98% overall agreement between the results obtained by the 2 methods. Of a total of 424 drug tests, only 8 drug results did not agree, mostly in the case of streptomycin. This new procedure was rapid, with 87% of the test results reportable within 4 days and 98% reportable within 5 days as compared to the usual 3 wk required with the conventional indirect susceptibility test method. The rapid radiometric method seems to have the potential for routine laboratory use and merits further investigations.


Accession: 005395612

PMID: 6787076



Related references

Primary isolation preliminary identification and drug susceptibility testing of the mycobacterium tuberculosis complex by a rapid radiometric method. Bulletin of the International Union Against Tuberculosis 57(1): 54, 1982

Rapid radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Research in Microbiology 140(4-5): 301-309, 1989

Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. Journal of Clinical Microbiology 18(3): 689-696, 1983

Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis. Nature Medicine 3(4): 465-468, 1997

Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method. Diagnostic Microbiology and Infectious Disease 10(1): 41-48, 1988

Evaluation of a radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Antimicrobial Agents and ChemoTherapy 30(6): 852-855, 1986

A combined detection and drug susceptibility testing of mycobacterium tuberculosis in smear positive sputum samples using biphasic vial radiometric method. Indian Journal of Medical Research 83(FEB): 123-135, 1986

Rapid radiometric susceptibility testing of Mycobacterium tuberculosis. American Review of Respiratory Disease 117(4): 631-637, 1978

Rapid susceptibility testing of Mycobacterium tuberculosis by a radiometric technique. Journal of Antimicrobial ChemoTherapy 10(4): 351-354, 1982

Rapid drug susceptibility testing of Mycobacterium tuberculosis by a fluorescence quenching method. Abstracts of the General Meeting of the American Society for Microbiology 93(0): 172, 1993