+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts

, : Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts. Journal of Chromatography 283: 37-46

A model proposed for the retention of proteins on high-performance ion-exchange chromatographic supports was further investigated via the use of NaCl vs. MgCl2 as the chromatographic displacing salt. Evaluations of the Z term [number of charged groups involved in the adsorption/desorption process] generated by this model were carried out using selected proteins on a strong anion-exchange support in an effort to define its meaning more clearly. A positive correlation between Z and protein retention was found. Higher values for ZMgCl2 were attributed to enhanced ionization of protein functional groups of Mg2+. Findings suggest that Z could be more precisely defined as the number of electrostatic interactions between the protein and the ion-exchange support.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005395642

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ahearn J.N.; Baimai V., 1987: Cytogenetic study of three closely related species of hawaiian drosophila. Three allopatric species from the Hawaiian islands, Drosophila bostrycha (Molokai), D. affinidisjuncta (West Maui), and D. disjuncta (East Maui), are extremely similar in morphology but differ in metaphase chromosomes, by the amount and distributi...

Kale, OO., 1981: The effect of drug treatment on the pattern of emergence of Onchocerca volvulus microfilariae from skin flaps. A controlled study was performed to determine the effect of some known anti-filarial drugs on the emergence pattern of O. volvulus microfilariae from skin snips from humans into saline. Before treatment, 81.7-92.1% of microfilariae emerge in all t...

Friedman, M., 1991: Putting zip back into yogurt sales. New yoghurt products launched in the USA include: fat-free yoghurts sweetened with aspartame (NutraSweet); yoghurts for the children's sector; yoghurt topped with a container of dried food, e.g. museli and chocolate fudge crunch; yoghurt beve...

Guo, Hua; Tittle, Thomas V.; Allen, Hamis; Maziarz, Richard T., 1998: Brefeldin A-mediated apoptosis requires the activation of caspases and is inhibited by Bcl-2. Brefeldin A (BFA) has recently been shown to induce apoptosis in human tumor cells in a p53-independent fashion. In this study, BFA-induced apoptosis was analyzed in the human Jurkat T-cell line. Apoptosis occurred 8 h after treatment with BFA and...

Dobson, W. D.; Christ, P., 2000: Structural change in the U.S. dairy industry: growth in scale, regional shifts in milk production and processing, and internationalism. Structural changes in the US dairy industry from the early 1980s to the late 1990s included familiar increases in concentration, industry adjustments to serve large supermarkets, the emergence of two national fluid milk firms (Suiza Foods and Dean...

Suslick, Kenneth S.; Rakow, Neal A.; Sen, Avijit, 2002: Colorimetric artificial nose having an array of dyes and method of artificial olfaction. The present invention involves an artificial nose having an array comprising at least a first dye and a second dye in combination and having a distinct spectral response to an analyte. In one embodiment, the first and second dyes are from the grou...

Grillo M.; Weisner D.; Franck A.; Hirsch W., 1983: Enlarging diagnostic possibilities in gynecological oncology with computed tomography. Archives of Gynecology 235(1-4): 150-151

Ichikawa, Yoshihito; Lemon, Stephen J.; Wang, Shuhe; Franklin, Barbara; Watson, Patrice; Knezetic, Joseph A.; Bewtra, Chhanda; Lynch, Henry T., 1999: Microsatellite instability and expression of MLH1 and MSH2 in normal and malignant endometrial and ovarian epithelium in hereditary nonpolyposis colorectal cancer family members. Mismatch repair deficiency is a characteristic molecular finding in hereditary nonpolyposis colorectal cancer (HNPCC), and has been demonstrated in both colorectal cancers and benign adenomas. Endometrial and ovarian cancers are common extracoloni...

Davies, H. V.; Chapman, J. M., 1979: The control of food mobilisation in seeds of Cucumis sativus L. I. The influence of embryonic axis and testa on protein and lipid degradation. The development of maximal rates of lipid and protein hydrolysis in cucumber cotyledons depended upon the removal of the testa and the presence of the embryonic axis. The testa appeared to exert at least part of its inhibitory influence by suppres...

Aisa C.; Alvarez Puebla R. A.; Blasco J.; Echeverria J. C.; Garrido J. J., 2005: Combining DLS, XRD, SEM-EDAX and EXAFS in the study of Zn(II) retention on a palygorskitic clay. Clay materials play a key role in determining the retention capacity of a soil, and are widely used in waste treatments. One of the most commonly used clays is palygorskite. The aim of this research is to determine the chemical species formed by Z...