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Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts


, : Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts. Journal of Chromatography 283: 37-46

A model proposed for the retention of proteins on high-performance ion-exchange chromatographic supports was further investigated via the use of NaCl vs. MgCl2 as the chromatographic displacing salt. Evaluations of the Z term [number of charged groups involved in the adsorption/desorption process] generated by this model were carried out using selected proteins on a strong anion-exchange support in an effort to define its meaning more clearly. A positive correlation between Z and protein retention was found. Higher values for ZMgCl2 were attributed to enhanced ionization of protein functional groups of Mg2+. Findings suggest that Z could be more precisely defined as the number of electrostatic interactions between the protein and the ion-exchange support.

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