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Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts


, : Evaluation of a retention model for high performance ion exchange chromatography using 2 different displacing salts. Journal of Chromatography 283: 37-46

A model proposed for the retention of proteins on high-performance ion-exchange chromatographic supports was further investigated via the use of NaCl vs. MgCl2 as the chromatographic displacing salt. Evaluations of the Z term [number of charged groups involved in the adsorption/desorption process] generated by this model were carried out using selected proteins on a strong anion-exchange support in an effort to define its meaning more clearly. A positive correlation between Z and protein retention was found. Higher values for ZMgCl2 were attributed to enhanced ionization of protein functional groups of Mg2+. Findings suggest that Z could be more precisely defined as the number of electrostatic interactions between the protein and the ion-exchange support.

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Related references

Malmquist, G.; Lundell, N., 1992: Characterization of the influence of displacing salts on retention in gradient elution ion-exchange chromatography of proteins and peptides. It has been shown earlier that the choice of displacing salt has a large effect on the retention in ion-exchange chromatography of proteins and peptides. The influence of different displacing salts cannot be predicted or quantitatively explained,...

Hodder, A.N.; Aguilar, M.I.; Hearn, M.T., 1989: High-performance liquid chromatography of amino acids, peptides and proteins. LXXXIX. The influence of different displacer salts on the retention properties of proteins separated by gradient anion-exchange chromatography. The influence of eight different displacer salts on the retention properties of four globular proteins, ranging in molecular weight from 14,000 to 43,000, was investigated by using the Mono-Q strong-anion-exchange resin as the stationary phase. Pr...

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