EurekaMag.com logo
+ Translate

Evaluation of a selective medium for isolation of Haemophilus pleuropneumoniae


, : Evaluation of a selective medium for isolation of Haemophilus pleuropneumoniae. Canadian Journal of Comparative Medicine 47(4): 445-450

Crystal violet, lincomycin, spectinomycin and bacitracin were evaluated as selective agents in media for isolation of H. pleuropneumoniae. No single antimicrobial agent or combination of 2 or more inhibited all non-Haemophilus strains (Escherichia coli, Pasteurella haemolytica, P. multocida, Streptococcus faecalis, S. equisimilis and Staphylococcus aureus) without marked suppression of 16 H. pleuropneumoniae strains. A medium containing 1 .mu.g/ml of crystal violet, 1 .mu.g/ml of lincomycin, 8 .mu.g/ml of spectinomycin and 128 .mu.g/ml of bacitracin inhibited 1 E. coli strain and the gram-positive strains while H. pleuropneumoniae strains were suppressed to a minor degree only. H. pleuropneumoniae was isolated on the selective medium on 3 occasions from the nose or pharynx of 2 of 8 experimentally inoculated pigs. H. pleuropneumoniae was recovered from the nose of only 2 pigs at necropsy and from tonsil of 1; the lower airways in most pigs and the lung lesions in all pigs were positive. There was no advantage to using the selective medium for the recovery of H. pleuropneumoniae at necroscopy from these 8 experimentally infected pigs, probably because other bacteria were absent or present in very low numbers in the tissues with H. pleuropneumoniae. The isolation rate on selective medium was higher than the rate on non-selective medium (P .ltoreq. 0.1; .chi.2 test) when the airways of slaughtered pigs were cultured. This was likely due to a high degree of contamination. Dry swabs placed in tryptone yeast extract with nicotinamide-adenine-dinucleotide gave a significantly higher recovery rate than commercial Culturette swabs in modified Stuart's transport medium. This difference was more marked after 24 h storage at room temperature, suggesting that the Culturette swab is not suitable for transporting samples to diagnostic laboratories for H. pleuropneumoniae culture. Fifty-one Haemophilus strains were isolated from 224 slaughtered pigs and identified as H. pleuropneumoniae serotype 1 (5 strains), serotype 5 (9 strains), serotype 7 (18 strains), minor group identical with strain 202 (4 strains) and nontypeable (15 strains).

(PDF 0-2 workdays service)

Accession: 005395674

PMID: 6230143

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Rosado-Vallado, M.; Quintero-Mármol, E.; Garcilazo, J.A., 1993: Evaluation of a selective medium with dilutions for the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp. in pigs. The aim of the present research was to evaluate the efficiency of a method used to enhance the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp. 134 samples of pneumonic lungs of swine were directly grown in blood agar medium, 120...

Rosado Vallado, M.; Quintero Marmol, E.; Garcilazo, J.A.A., 1994: Evaluation of a selective medium with sample dilution for the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp from swine. The aim of the present research was to evaluate the efficiency of the method used to enhance the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp. 134 samples of pneumonic lungs of swine were directly grown in blood agar medium, 12...

Taylor, D.C.; Cripps, A.W.; Clancy, R.L.; Murree-Allen, K.; Hensley, M.J.; Saunders, N.A.; Sutherland, D.C., 1990: Evaluation of a selective medium for the isolation and differentiation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory tract of chronic bronchitics. Respiratory tract specimens from chronic bronchitic patients were cultured for Haemophilus species on conventional chocolate agar and a modified sucrose medium in order to determine the accuracy of the new medium in differentiating Haemophilus inf...

Jacobsen, M.J.; Nielsen, J.P., 1995: Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar...

Klein, M.; Blazevic, D.J., 1970: Evaluation of a selective medium for isolation of Haemophilus from respiratory cultures. American Journal of Medical Technology 36(3): 97-106

Sturm, A.W., 1986: Isolation of Haemophilus influenzae and Haemophilus parainfluenzae from genital-tract specimens with a selective culture medium. A series of 2027 genital tract specimens was cultured for Haemophilus species on non-selective chocolate agar and on a selective medium (Choc-VBCA). The latter gave a significantly higher isolation rate. H. influenzae was isolated from 27 specimen...

O'Reilly, T.; Niven, D.F., 1986: Tryptone-yeast extract broth as a culture medium for Haemophilus pleuropneumoniae and Haemophilus parasuis to be used as challenge inocula. Haemophilus pleuropneumoniae and Haemophilus parasuis were grown in a liquid medium containing tryptone and yeast extract. Whereas culture turbidity during exponential growth of either organism was proportional to the number of viable cells, conti...

O'reilly T.; Niven D.F., 1986: Trypton yeast extract broth as a culture medium for haemophilus pleuropneumoniae and haemophilus parasuis to be used as challenge inocula. Haemophilus pleuropneumoniae and Haemophilus parasuis were grown in a liquid medium containing tryptone and yeast extract. Whereas culture turbidity during exponential growth of either organism was proportional to the number of viable cells, conti...

Rapp, V.J.; Ross, R.F.; Young, T.F., 1985: Characterization of Haemophilus spp. isolated from healthy swine and evaluation of cross-reactivity of complement-fixing antibodies to Haemophilus pleuropneumoniae and Haemophilus taxon "minor group". Of 30 sows from a herd believed to be free of Haemophilus pleuropneumoniae infection, 2 had complement-fixing antibodies to H. pleuropneumoniae serotype 5. Necropsy and microbiological examination of the two sows revealed no evidence of H. pleurop...

Csukas Z., 1980: New selective medium for the isolation of haemophilus spp. Acta Microbiologica Academiae Scientiarum Hungaricae 27(3): 220-221