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Evaluation of a semi automated pre standardized immuno fluorescence test system for detection of anti native dna antibodies


, : Evaluation of a semi automated pre standardized immuno fluorescence test system for detection of anti native dna antibodies. Journal of Clinical Microbiology 7(2): 219-222

An immunofluorescence-native (nDNA) antibody test system was compared with a radioimmunoassay procedure (FARR assay) for detecting anti-nDNA antibodies in human serum. Double-blind studies of split samples obtained from 236 patients showed an 80% correlation between the immunofluorescence-nDNA antibody test system and the radioimmunoassay procedure. Studies of sera from patients with known diagnoses showed positive nDNA antibody findings in biopsy-proven systemic lupus erythematosus only. The immunofluorescence-nDNA antibody test system provides a reliable, simple, and economically feasible alternative method for detecting nDNA antibodies that can be employed in any laboratory equipped with a fluorescence microscope.

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