EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a standard scrubbing method for the recovery of aerobic skin flora


, : Evaluation of a standard scrubbing method for the recovery of aerobic skin flora. Annales de l'Institut Pasteur Microbiology 138(3): 349-358

The most reliable method for sampling skin flora is still a matter of debate, although the subject is of importance in many fields ranging from clinical to basic research. For the evaluation of normal skin flora, Williamson and Kligman's scrub method is the most commonly used. In order to determine the value of this method, standardized for the evaluation of topical antimicrobial agents or anerobic skin flora, we compared its results to those obtained both from bacterial counts and from microscopic studies on biopsies performed at the same site. After different experiments had established the optimal experimental conditions on human pig and pig skin, a comparative study on six healthy subjects was carried out in the peri-umbilical area. The mean log numbers of aerobic bacteria in the shrub samples and in the biopsies were respectively, 3.1 and 2.9, which were not statistically significant. These numerical results were similar to those obtained by electron microscopy. Hence, the standard scrub method may be considered satisfactory for the recovery of aerobic bacteria from healthy human skin.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005395751

PMID: 3304351

DOI: 10.1016/0769-2609(87)90123-2

Download PDF Full Text: Evaluation of a standard scrubbing method for the recovery of aerobic skin flora


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Suske, G.; Enders, C.; Schlerf, A.; Kadenbach, B., 1988: Organization and nucleotide sequence of two chromosomal genes for rat cytochrome c oxidase subunit VIc: a structural and a processed gene. A rat genomic library was screened with a cDNA probe coding for rat liver cytochrome c oxidase subunit VIc (COX-VIc). Out of 16 clones mapped, four inserts were different and did not overlap. Two inserts that gave strong hybridization signals were...

Schwartz, S.; Sullivan, B.A.; Depinet, T.W.; Becker, L.A.; Zinn, A.B.; Stallard, J.R.; Micale, M.A.; Sekhon, G.S.; Vine, D.; Kaffe, S., 1994: Marker chromosomes lacking alpha-satellite DNA A new intriguing class of abnormalities. American Journal of Human Genetics 55(3 SUPPL ): A37

Machuca, A.; Sanchez, R.; Lescaille, M.; Basill, J.; Murgado, G.; Garcel, A.; Moya, R., 1998: Use of the aquatic plant Elodea densa on the feeding of laying hens. II. In a randomized block design with 6 blocks and 2 treatments per block, 3312 White Leghorn laying hens, 56 weeks old, were fed on diets containing 0 or 15% Elodea densa for 55 days. Egg production and quality were similar between groups. Egg yolk c...

Olthof, T.H., 1983: Effect of Plant Age and Transplanting Damage on Sugar Beets infected by Heterodera schachtii. Glasshouse and growthroom experiments showed that sugarbeet seeds sown into nematode-infested soil are damaged much more heavily by H. schachtii juveniles than seeds inoculated with H. schachtii immediately following sowing. There was no increase...

Kelly, J.W.; McNamara, T.P., 2008: Response mode differences in perspective taking: differences in representation or differences in retrieval?. Three experiments explored whether response mode differences in perspective taking result from different spatial representations or different retrieval processes. Participants learned object locations and then, while blindfolded, pointed to or ver...

Yang, K.; Wang, S.; Chang, K.O.; Lu, S.; Saif, L.J.; Greenberg, H.B.; Brinker, J.P.; Herrmann, J.E., 2001: Immune responses and protection obtained with rotavirus VP6 DNA vaccines given by intramuscular injection. Intramuscular (i.m.) injection of murine VP6 DNA vaccines raised high titers of rotavirus-specific serum IgG and IgA antibodies in BALB/c mice. A Th1-like antibody response was generated based on the ratio of serum IgG2a to IgG1 antibodies. Rotavi...

Lu, Y.H.; Qian, H.Z.; Hu, A.Q.; Qin, X.; Jiang, Q.W.; Zheng, Y.J., 2014: Seasonal pattern of hepatitis E virus prevalence in swine in two different geographical areas of China. We studied seasonal patterns of swine hepatitis E virus (HEV) infection in China. From 2008 to 2011, 4200 swine bile specimens were collected for the detection of HEV RNA. A total of 92/2400 (3·83%) specimens in eastern China and 47/1800 (2·61%)...

Delage, Y.; Erlinger, S.; Duval, M.; Bpenhamou, J.P., 1975: Influence of dehydrocholate and taurocholate on bromsulphthalein uptake, storage, and excretion in the dog. The influence of dehydrocholate on bromsulphtalein relative-storage capacity, bilary transport maximum (Tm), and fractional transfer rates between plasma, liver and bile have been studied in unanaesthetized dogs. In six dogs, storage capacity, Tm,...

Baron, M.; Hudson, M.; Tatibouet, Sène.; Steele, R.; Lo, E.; Gravel, S.; Gyger, Gève.; E.S.yegh, T.; Pope, J.; Fontaine, A.; Masetto, A.; Matthews, D.; Sutton, E.; Thie, N.; Jones, N.; Copete, M.; Kolbinson, D.; Markland, J.; Nogueira-Filho, G.; Robinson, D.; Gornitsky, M., 2015: The Canadian systemic sclerosis oral health study II: the relationship between oral and global health-related quality of life in systemic sclerosis. Both oral and global health-related quality of life (HRQoL) are markedly impaired in SSc. In this study we aimed to determine the degree of association between oral HRQoL and global HRQoL in SSc. Subjects were recruited from the Canadian Scleroder...

Dovydaitis, V.; Baliuneviciute, Z.; Kemesyte, E., 1973: Comparison of peas, vetch, barley and oats grown for grain. In trials in 1959-61, peas, barley, oats, pea/barley and pea/oat mixtures gave average seed and/or grain yields of 2.17, 3.95, 3.01, 4.18 and 3.04 t/ha, respectively. The plant height in barley and oats increased and that in peas decreased in mixt...