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Evaluation of a standard scrubbing method for the recovery of aerobic skin flora

, : Evaluation of a standard scrubbing method for the recovery of aerobic skin flora. Annales de l'Institut Pasteur Microbiology 138(3): 349-358

The most reliable method for sampling skin flora is still a matter of debate, although the subject is of importance in many fields ranging from clinical to basic research. For the evaluation of normal skin flora, Williamson and Kligman's scrub method is the most commonly used. In order to determine the value of this method, standardized for the evaluation of topical antimicrobial agents or anerobic skin flora, we compared its results to those obtained both from bacterial counts and from microscopic studies on biopsies performed at the same site. After different experiments had established the optimal experimental conditions on human pig and pig skin, a comparative study on six healthy subjects was carried out in the peri-umbilical area. The mean log numbers of aerobic bacteria in the shrub samples and in the biopsies were respectively, 3.1 and 2.9, which were not statistically significant. These numerical results were similar to those obtained by electron microscopy. Hence, the standard scrub method may be considered satisfactory for the recovery of aerobic bacteria from healthy human skin.

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Accession: 005395751

PMID: 3304351

DOI: 10.1016/0769-2609(87)90123-2

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Suske, G.; Enders, C.; Schlerf, A.; Kadenbach, B., 1988: Organization and nucleotide sequence of two chromosomal genes for rat cytochrome c oxidase subunit VIc: a structural and a processed gene. A rat genomic library was screened with a cDNA probe coding for rat liver cytochrome c oxidase subunit VIc (COX-VIc). Out of 16 clones mapped, four inserts were different and did not overlap. Two inserts that gave strong hybridization signals were...

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Lu, Y.H.; Qian, H.Z.; Hu, A.Q.; Qin, X.; Jiang, Q.W.; Zheng, Y.J., 2014: Seasonal pattern of hepatitis E virus prevalence in swine in two different geographical areas of China. We studied seasonal patterns of swine hepatitis E virus (HEV) infection in China. From 2008 to 2011, 4200 swine bile specimens were collected for the detection of HEV RNA. A total of 92/2400 (3·83%) specimens in eastern China and 47/1800 (2·61%)...

Delage, Y.; Erlinger, S.; Duval, M.; Bpenhamou, J.P., 1975: Influence of dehydrocholate and taurocholate on bromsulphthalein uptake, storage, and excretion in the dog. The influence of dehydrocholate on bromsulphtalein relative-storage capacity, bilary transport maximum (Tm), and fractional transfer rates between plasma, liver and bile have been studied in unanaesthetized dogs. In six dogs, storage capacity, Tm,...

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Dovydaitis, V.; Baliuneviciute, Z.; Kemesyte, E., 1973: Comparison of peas, vetch, barley and oats grown for grain. In trials in 1959-61, peas, barley, oats, pea/barley and pea/oat mixtures gave average seed and/or grain yields of 2.17, 3.95, 3.01, 4.18 and 3.04 t/ha, respectively. The plant height in barley and oats increased and that in peas decreased in mixt...