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Evaluation of a synthetic oligonucleotide probe for diagnosis of Plasmodium falciparum infections


, : Evaluation of a synthetic oligonucleotide probe for diagnosis of Plasmodium falciparum infections. American Journal of Tropical Medicine and Hygiene 35(5): 912-920

A radiolabeled synthetic oligonucleotide was evaluated as a diagnostic probe specific for Plasmodium falciparum using blood samples lysed directly on nitrocellulose filters. The probe technique successfully diagnosed malaria in experimentally infected chimpanzees that had 0.001% parasitemias (50 parasites/microliter) as determined by blood smears, and in 1 chimpanzee whose blood smear was negative, but whose blood was culture-positive for P. falciparum. In a double blind study of 50 patient samples from the Philippines, the probe results correlated well with blood smear results when the autoradiographs were read after 4-8 hr exposure. The results indicate that the oligonucleotide probe may be useful in the rapid and specific diagnosis of P. falciparum infection.

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Accession: 005395773

PMID: 3532848

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