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Evaluation of a test kit for the rapid and simple colorimetric measurement of angiotensin i converting enzyme ec 3.4.15.1 in serum


Journal of Clinical Chemistry and Clinical Biochemistry 21(12): 845-850
Evaluation of a test kit for the rapid and simple colorimetric measurement of angiotensin i converting enzyme ec 3.4.15.1 in serum
A recently introduced color test kit for the determination of [human] serum angiotensin I-converting enzyme catalytic activity was evaluated. p-Hydroxyhippuric acid, liberated from p-hydroxyhippuryl-L-histidyl-L-leucine by angiotensin I-converting enzyme, is converted into a quinoneimine dye with an absorption maximum at 505 nm. The procedure shows excellent linearity over the whole range of catalytic activities found in serum. Intra- and inter-assay coefficients of variation are 2-5 and 7-10%, respectively. Correlation with a modified Cushman-Cheung method currently used in this laboratory is good, with r = 0.985 and a regression equation of y (color kit) = 0.423x (Cushman-Cheung) + 0.765. Hb, lipids, bilirubin and prednisone do not interfere but uric acid in concentrations > 600 .mu.mol/l does. No extraction step is required. The assay is very rapid and more than 20 samples can be determined in 1 h.

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Accession: 005395798



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