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Evaluation of a testicular sperm head counting technique using rats exposed to di methoxyethyl phthalate glycerol alpha mono chlorohydrin epi chlorohydrin formaldehyde or methyl methanesulfonate






Archives of Toxicology 53(1): 71-78

Evaluation of a testicular sperm head counting technique using rats exposed to di methoxyethyl phthalate glycerol alpha mono chlorohydrin epi chlorohydrin formaldehyde or methyl methanesulfonate

Single dosages of dimethoxyethylphthalate (DMEP, 1000-2000 mg/kg), glycerol .alpha.-monochlorohydrin (GMCH, 50 mg/kg), epichlorohydrin (ECH, 25 and 50 mg/kg), formaldehyde (FA, 100 and 200 mg/kg), and methyl-methanesulfonate (MMS, 100-400 mg/kg) were administered orally to 10 wk old male Wistar rats. The rats were necropsied on the 11th day following dosing. The testes were weighed, homogenized and sonicated; numbers of sperm heads (total and abnormal) were counted and percentage sperm head abnormalities were calculated. Testes weights were significantly reduced only in rats exposed to 1500 and 2000 mg DMEP/kg. Compared with controls, there were significant increases in the incidence of abnormal sperm at all dose levels of MMS and the higher dose levels of DMEP (1500 and 2000 mg/kg), ECH and FA. No toxicologically significant effects upon total sperm counts were seen following the oral administration of any of the 5 chemicals tested. However, an additional group of rats given 100 mg MMS/kg i.p. showed significant reductions in testes weight and total sperm head counts compared with control animals. Testicular sperm head counting is a useful tool in the detection of selective adverse effects of chemicals [occupational and environmental toxicants] chemicals upon testicular sperm but requires further evaluation.


Accession: 005395804



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