+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of absorption with limulus amoebocyte lysate to remove contaminating endo toxin from interferon and lymphokine preparations

, : Evaluation of absorption with limulus amoebocyte lysate to remove contaminating endo toxin from interferon and lymphokine preparations. Journal of Immunological Methods 66(1): 103-112

.alpha. and .beta. human interferon (IFN) preparations and lymphokines (supernatants of PHA[phytohemagglutinin]-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in 2. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005395876

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Garner, Peter R., 1995: Glucose metabolism assessment in pregnancy. Objectives: To review the literature regarding screening and definitive testing for Gestational Diabetes (GDM) and to assess whether treatment after identification changes perinatal/neonatal outcome. Data Resources: Directed medline searches. Resu...

Richie, John P.Jr; Leutzinger, Yvonne; Parthasarathy, Saudhamini; Malloy, Virginia; Orentreich, Norman; Zimmerman, Jay A., 1994: Methionine restriction increases blood glutathione and longevity in F344 rats. Little is known about the biochemical mechanisms responsible for the biological aging process. Our previous results and those of others suggest that one possible mechanism is based on the loss of glutathione (GSH), a multifunctional tripeptide pre...

Schormuller, J.; Huth, H., 1954: Biochemistry of cheese ripening. 7. Phosphorus balances in ripening sour milk cheese. For previous parts see Absts. 4263, Vol. 24; 220, Vol. 25. Phosphorus fractions were estimated in sour milk cheese at different stages of ripening, and the P content in several cases was related to that of N. The P: N ratio in the total mass of th...

Czapla, J., 2000: The effects of nitrogen and potassium fertilization on the yield and quality of orchard grass grown on arable land: II. Content of: total protein, soluble sugars, crude fibre and digestibility. During a three-year experiment on arable land of very good rye soil complex, the influence of differential nitrogen rates (60, 120, 180, 240 and 300 kg N/ha) and potassium (100 and 200 kg K/ha) on the contents of total protein, sugars soluble in w...

Laing, Chris, M.; Roberts, Rhys; Lightstone, Liz; Graham, Alison; Cook, Terry, H.; Summers, Shaun; Pusey, Charles, D., 2007: Interactive case report - A patient with suspected miscarriage is found to have hypertension, renal failure, and thrombocytopenia: case presentation. BMJ 334(7608): 1372

Pospisil, Z; Mensik, J; Tumova, B; Stumpa, A; Cerny, M., 1973: Experimental infection of colostrum-deprived, specific-pathogen free piglets with A. Zentralblatt fur Veterinarmedizin: Reihe B: 20 (2) 139-152

Cypher, Brian L.; Warrick, Gregory D., 1993: Use of human-derived food items by urban kit foxes. Transactions of the Western Section of the Wildlife Society, 29: 34-37

Ahlmann, K.; Svendsen, J.; Bengtsson, A. C., 1983: Rectal temperature of the newborn pig measured under different environmental conditions. The rectal temperature (RT) of piglets during the period immediately after birth was measured in the offspring of a herd of 90 sows. RT declined greatly in the first 15-30 minutes after birth, after which it began to increase, and stabilized after...

Morozova G.I.; Dobretsov G.E.; Barenboim G.M., 1982: Recording of surface charge changes in erythrocytes and model membranes by means of fluorescent probe. When staining intact erythrocytes with DSM [4-(n-dimethylaminosteryl)-1-methylpyridine], no quenching of its fluorescence with hems in the membrane was observed. The positively charged fluorescent probe DSM was proposed for estimating a relative c...

Khusainova, N.Z., 1955: Acclimatization of invertebrates as fodder for fish in Balkhash. Uchenkazan. Univ. biol. agric. Alma-Ata: 118-130