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Evaluation of absorption with limulus amoebocyte lysate to remove contaminating endo toxin from interferon and lymphokine preparations


, : Evaluation of absorption with limulus amoebocyte lysate to remove contaminating endo toxin from interferon and lymphokine preparations. Journal of Immunological Methods 66(1): 103-112

.alpha. and .beta. human interferon (IFN) preparations and lymphokines (supernatants of PHA[phytohemagglutinin]-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in 2. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

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