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Evaluation of polyacrylamide gel electrophoresis bioassay and dot blot methods for the survey of potato spindle tuber viroid



Evaluation of polyacrylamide gel electrophoresis bioassay and dot blot methods for the survey of potato spindle tuber viroid



Canadian Plant Disease Survey 65(2): 61-63



Three methods (e.g. polyacrylamide gel electrophoresis (PAGE), bioassay using Solanum berthaultii and dot-blot) were compared for the detection of potato spindle tuber viroid (PSTV). Composite samples ranging in dilution from 1:3 to 1:500 (infected discs: healthy discs) were used. Extracted nuclei acid was used for all the tests. PAGE could not detect PSTV beyond 1:10 dilution whereas both bioassay and dot-blot detected PSTV reliably up to 1:300 dilution. Using bioassay and dot-blot, PSTV was surveyed in 103 tablestock fields of potato. Tablestock fields of potato cultivars Kennebec, Russet Burbank, Shepody and Superior planted with seed grade Elite II to Certified were surveyed. No PSTV was found in any of the 618 composite samples tested, which represented 51,500 leaves. Freedom of the seed potato crop from PSTV was thus confirmed by laboratory tests.

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Accession: 005400569

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