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Evaluation of self prepared enzyme immunoassay kit for detection of alpha fetoprotein

Evaluation of self prepared enzyme immunoassay kit for detection of alpha fetoprotein

Chinese Journal of Microbiology & Immunology (Taipei) 19(1): 52-60

A "sandwich" technique of enzyme immunoassay (EIA) kit for human alpha-fetoprotein has been developed by using commercially available reagents. The assay range is 0.5-60 ng/ml. Within-run coefficients of variation (C.V.) for 20 determination at three different concentrations were 4.4-10.3%, and between-run C.V. were 4.9-10.8%. The sensitivity and specificity of the kit has been compared with those of two commercially available kits (Abbott and Roche r1 = 0.991, r2 = 0.989). This kit has some advantages over currently available kits. The assay is incubated in room temperature, no waterbath is required. The solid phase is dry store, it have long shelf life. The kit is inexpensive to operate, and it is suitable for any laboratory.

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Accession: 005401395

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PMID: 2434285

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