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Evaluation of the standard total coliform most probable number test for the biological examination of water pollution



Evaluation of the standard total coliform most probable number test for the biological examination of water pollution



Okayama Igakkai Zasshi 90(9-10): 1309-1318



The standard total coliform MPN [most probable number] test is widely used as a biological indicator of water pollution. Applications of the test to various field studies resulted in much higher cell numbers than expected probably because bacteria of the coliform group were not always derived from human and animal feces. To examine this possibility, bacteria which were detected by this method were isolated and identified. The bacteria designated as coliform group included not only human- and animal-derived bacteria but also bacterial species which apparently derived from plants, soil or water, e.g., Erwinia, Aeromonas, Serratia, Citrobacter. A method is required by which only bacteria associated with human and animal feces can be estimated quantitatively.

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