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Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology elisa and slot blot hybridization



Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology elisa and slot blot hybridization



Plant Disease 71(8): 714-719



Enzyme-linked immunosorbent assay (ELISA) and a slot-blot hybridization assay were developed to evaluate a large number of accessions of wild wheat species, in conjunction with a symptomatology assay, for resistance to wheat streak mosaic virus (WSMV). No resistance was found among the Triticum or the Aegilops species tested. Five wheat/wheatgrass (Thinopyrum = Agropyron species) amphiploids were found resistant to WSMV. Nine registered germ plasms resistant to WSMV were evaluated; eight were immune to the virus and one gave a susceptible reaction. Both ELISA and slot-blot hybridization assays were sensitive in virus detection. The three assays showed good correlation.

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