Evidence for a glycosidic linkage between chitin and glucan in the cell wall of Candida albicans
Surarit, R.; Gopal, P.K.; Shepherd, M.G.
Journal of General Microbiology 134(6): 1723-1730
ISSN/ISBN: 0022-1287 PMID: 3065455 DOI: 10.1099/00221287-134-6-1723
The alkali-insoluble glucan was isolated from regenerating spheroplats and intact cells of Candida albicans. Sequential enzymic hydrolysis of this fraction by Zymolyase 100T and purified chitinase and subsequent gel filtration produced a fraction which was enriched in glycosaminoglycans. This fraction was analysed by partial acid hydrolysis, TLC and GLC-MS. The GLC-MS peaks identified included 2,3,4,6-tetra-O-methylglucitol acetate and 2,3,4-tri-O-methylglucital acetate of .beta.-1,6-glucan and the 3,6-di-O-methyl-2-N-methylglucosaminititol acetate of chitin. In addition, 3-O-methyl-2-N-methylglucosaminitol acetate was identified, which indicated a branch point in chitin. These data provide evidence for a covalent linkage between chitin and .beta.-(1,6)-glucan through a glycosidic linkage at position 6 of N-acetylglucosamine and position 1 of the glucose in the glucan.