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Evidence for variation in the number of functional gene copies at the alpha amanitin resistant locus in chinese hamster cell lines



Evidence for variation in the number of functional gene copies at the alpha amanitin resistant locus in chinese hamster cell lines



Journal of Cellular Physiology 97(3 Part 2): 461-468



The hypothesis of functional hemizygosity was examined for the .alpha.-amanitin resistant (AmaR, a codominant marker) locus in a series of Chinese hamster cell lines. AmaR mutants were obtained from different cell lines, e.g., CHO, CHW, M3-1 and CHO-Kl, at similar frequencies. After fractionation of different RNA polymerase activities in the extracts by chromatographic procedures, the sensitivity of the mutant RNA polymerase II towards .alpha.-amanitin was determined. While all of the RNA polymerase II activity in mutant CHO and CHO-Kl lines became resistant to .alpha.-amanitin inhibition, only about 50% of the activity is highly resistant in AmaR mutants CHW and M3-1 cell lines. The remaining activity in the latter cell lines shows .alpha.-amanitin sensitivity similar to that of the wild type enzyme. This behavior is similar to that observed with a 1:1 mixture of resistant and sensitive enzymes from CHO cells. While only 1 functional copy of the gene affected by .alpha.-amanitin is present in CHO and CHO-Kl cells, 2 copies of this gene are apparently functional in the CHW and M3-1 cell lines.

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Accession: 005410878

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