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Evidence in vitro of hydrolysis of estrone and estradiol 17 beta 3 sulfate by the uterus of pregnant guinea pigs


, : Evidence in vitro of hydrolysis of estrone and estradiol 17 beta 3 sulfate by the uterus of pregnant guinea pigs. Comptes Rendus des Seances de l'Academie des Sciences Serie III Sciences de la Vie 292(9): 609-612

The metabolism, in vitro, of [6,7-3H]-estrone-3-sulfate and [6,7-3H]-estradiol-17.beta.-3-sulfate by the uteri of pregnant female guinea pigs was studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the radioactivity was recovered in the unconjugated fractions after 1 h of incubation. Glucuronide estrogens were also recovered by the rate of synthesis was very low (3.6% after 1 h). Estrone and estradiol-17.beta. were identified in unconjugated, glucuro and sulfo-conjugated fractions after incubation with the [3H]-estrogen sulfates.

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