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Evidence in vitro of hydrolysis of estrone and estradiol 17 beta 3 sulfate by the uterus of pregnant guinea pigs


, : Evidence in vitro of hydrolysis of estrone and estradiol 17 beta 3 sulfate by the uterus of pregnant guinea pigs. Comptes Rendus des Seances de l'Academie des Sciences Serie III Sciences de la Vie 292(9): 609-612

The metabolism, in vitro, of [6,7-3H]-estrone-3-sulfate and [6,7-3H]-estradiol-17.beta.-3-sulfate by the uteri of pregnant female guinea pigs was studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the radioactivity was recovered in the unconjugated fractions after 1 h of incubation. Glucuronide estrogens were also recovered by the rate of synthesis was very low (3.6% after 1 h). Estrone and estradiol-17.beta. were identified in unconjugated, glucuro and sulfo-conjugated fractions after incubation with the [3H]-estrogen sulfates.


Accession: 005411024

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Related references

Adessi, G.L.; Nhuan, T.Q.; Goutte-Coussieu, C.; Jayle, M.F., 1981: Evidence, in vitro, of estrone and estradiol-17 beta 3-sulfoconjugate hydrolysis in the uterus of pregnant guinea pigs. The metabolism, in vitro, of [6,7-3H]-oestrone-3-sulfate and [6,7-3H]-oestradiol-17beta-3-sulfate by the uteri of pregnant female Guinea-Pigs has been studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the ra...

Tran Quang Nhuan; Adessi, G.L., 1979: A comparison of the in vitro metabolism of estrone, estradiol-17 beta and estrone-3-sulfate by the livers of young virgin female, pregnant and female fetal guinea-pigs. The metabolism, in vitro, of [3H]-estrone, [3H]-estradiol-17 beta and [13H]-estrone-3-sulfate by the livers of pregnant, young virgin female and female fetus guinea-pigs has been compared using 900 g supernatants and microsomes. The ability of the...

Adessi G.L.; Nhuan T.Q.; Vingler P., 1982: In vivo and in vitro metabolism of estrone and estradiol 17 beta and their 3 sulfates in pregnant female guinea pigs a plausible pre hormone role of estrogen sulfates in the maternal uterus. The amounts of unconjugated and conjugated estrogens, in the amniotic fluid and the plasma of mother and fetus guinea-pigs, were determined by radioimmunoassay. In plasma, the major compounds were estrogen sulfates with the highest values being th...

Urabe, M.; Chetrite, G.; Pasqualini, J., R., 1993: Transformation of estrone, estradiol, and estrone sulfate in uterine and vaginal isolated cells of fetal guinea pigs: Effect of various antiestrogens in the conversion of estrone sulfate to estradiol. The metabolism of physiological concentrations (5 times 10-19 M) of (3H)estrone (E-1), (3H)estradiol (E-2), and (3H)estrone sulfate (E-1S) was studied in isolated fetal uterine and vaginal cells of guinea pigs in culture. After 24 hours of incubat...

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D.Wever F., 1969: In vitro stimulation of protein synthesis in uterine microsomal supernatant by estrone sulfate metab rat rabbit estrone metab 17 beta estradiol metab estriol metab 17 beta estradiol 3 sulfate metab estradiol 17 beta sulfate metab estradiol 3 17 beta di sulfate metab. Endocrinology: 901-907

Myking O.; Laerum O.D., 1986: Hepatic estrone sulfotransferase activity and plasma levels of estrone sulfate estrone and estradiol in male guinea pigs with fatty liver. Fatty liver was induced in male guinea pigs by combined administration of carbon tetrachloride and azathioprine from the 3rd to 15th week of age. The final body and liver weights were significantly lower than in the control animals, whereas the li...

Adessi, G.; Goutte-Coussieu, C.; Nhuan, T.Q.; Jayle, M.F., 1977: In vitro metabolism of (6,7-H3)estrone and of (6,7-H3) estradiol by the liver in pregnant guinea pigs. Slices of pregnant guinea pig liver were incubated with (6,7-3H)estrone and with (6,7-3H)estradiol. Free, glucuro- and sulfo-conjugated fractions were isolated by specific extraction and hydrolysis. The radioactivity distribution in these 3 fracti...

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