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Evidence indicating independent assortment of framework and complementarity determining segments of the variable regions of rabbit light chains


, : Evidence indicating independent assortment of framework and complementarity determining segments of the variable regions of rabbit light chains. Journal of Experimental Medicine 152(1): 72-84

Amino acid sequences of rabbit L chains show considerable evidence of independent assortment of framework (FR) and complementarity-determining (CDR) segments. Apparently, they are coded for by independent genetic units (minigenes) and individual L chanins are assembled somatically by recombining these units. Identical FR sets with multiple members generally comprise chains with different specificities; identical CDR sets tend to have chains of a single specificity. A J segment, which, by analogy with mouse L chains, is made up of the last 2 residues of CDR3 plus all of FR4, contained 18 different sets and could contribute to diversity generated by CDR3. The longest segment, FR3, had a very large number of sets. The number of sets could be substantially reduced by permitting FR3 to be formed by 2 independently assorting segments comprising residues 57-68 and 69-88.

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Kabat, E.A.; Wu, T.T.; Bilofsky, H., 1980: Evidence indicating independent assortment of framework and complementarity-determining segments of the variable regions of rabbit light chains. Delineation of a possible J minigene. Amino acid sequences of rabbit light chains show considerable evidence of independent assortment of framework (FR) and complementarity-determining (CDR) segments. This suggests that they are coded for by independent genetic units (minigaenes) and...

Kabat, E.A.; Wu, T.T.; Bilofsky, H., 1979: Evidence supporting somatic assembly of the DNA segments (minigenes), coding for the framework, and complementarity-determining segments of immunoglobulin variable regions. Two sets of apparently conflicting data on the genes coding for the variable region are being accumulated. One suggests that the sets of nucleotides coding for the framework segments of immunoglobulin light and heavy (VL and VH) chains assort inde...

Riesen W.F., 1981: Linkage relationship between complementarity determining and framework segments of human immuno globulin variable regions. Steinberg, C M And I Lefkovits (Ed ) The Immune System, Vol 2 The Present; Festschrift in Honor Of Niels Kaj Jerne on The Occasion Of His 70th Birthday Xix+470p S Karger: Basel, Switzerland; New York, N Y , Usa Illus P141-146

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