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Evidence of 2 rna polymerase activities in euglena gracilis chloroplasts


, : Evidence of 2 rna polymerase activities in euglena gracilis chloroplasts. Journal of Biological Chemistry 259(23): 14880-14887

Two types of RNA polymerase activity were isolated from Euglena gracilis chloroplasts and compared. One polymerase is tightly bound to chloroplast DNA; this complex is called the transcriptionally active chromosome (TAC). The other activity is found in a soluble extract of E. chloroplasts. The soluble extract is dependent on an exogenous DNA template for activity. The 2 activities can be isolated in 2 distinct subchloroplast fractions from a single chloroplast preparation. The soluble extract is selective for transcription of tRNA genes, whereas the TAC is selective for rRNA genes. TAC and the soluble extract respond differently to KCl and Mg2+. The soluble extract is sensitive to heparin, and TAC is resistant. The 2 activities have different temperature optima. This evidence suggests that E. chloroplasts have at least 2 distinct RNA polymerase activities.

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Related references

Greenberg, B.M.; Narita, J.O.; DeLuca-Flaherty, C.; Gruissem, W.; Rushlow, K.A.; Hallick, R.B., 1984: Evidence for two RNA polymerase activities in Euglena gracilis chloroplasts. Two types of RNA polymerase activity were isolated from Euglena gracilis chloroplasts and compared. One polymerase is tightly bound to chloroplast DNA; this complex is called the transcriptionally active chromosome (TAC) (Rushlow, K. E., Orozco, E...

Greenberg, BM.; Narita, JO.; Deluca-Flaherty, C.; Gruissem, W.; Rushlow, KA.; Hallick, RB., 1984: Evidence for two RNA polymerase activities in Euglena gracilis. Two types of RNA polymerase activity were isolated from Euglena gracilis chloroplasts and compared. One polymerase is tightly bound to chloroplast DNA; this complex is called the transcriptionally active chromosome (TAC). The other activity is fou...

Schiemann, J.; Wollgiehn, R.; Parthier, B., 1978: Dna dependent rna polymerase in euglena gracilis broken chloroplasts. The DNA-dependent RNA polymerase activity of isolated, purified, osmotically ruptured Euglena chloroplasts was studied with regard to enzymatic parameters, RNA product analysis and sensitivity against transcription inhibitors. The incorporation of...

Narita, JO., 1986: Characterization of ribosomal RNA specific polymerase from the chloroplasts of Euglena gracilis. Dissertation Abstracts International B Sciences and Engineering, 468: 2530

Farmerie, WG., 1981: Sequence determination of the Euglena gracilis chloroplast and cytoplasmic aspartic acid tRNAs as a further investigation into the evolutionary origins of chloroplasts and Euglena gracilis. Dissertation Abstracts International B Sciences and Engineering, 418: 3007

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Brandt, P., 1981: Evidence for regulative transactions between the nucleocytoplasm and the chloroplasts in Euglena gracilis. Berichte der Deutschen Botanischen Gesellschaft 94(3): 419-433