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Evidence of 2 rna polymerase activities in euglena gracilis chloroplasts

, : Evidence of 2 rna polymerase activities in euglena gracilis chloroplasts. Journal of Biological Chemistry 259(23): 14880-14887

Two types of RNA polymerase activity were isolated from Euglena gracilis chloroplasts and compared. One polymerase is tightly bound to chloroplast DNA; this complex is called the transcriptionally active chromosome (TAC). The other activity is found in a soluble extract of E. chloroplasts. The soluble extract is dependent on an exogenous DNA template for activity. The 2 activities can be isolated in 2 distinct subchloroplast fractions from a single chloroplast preparation. The soluble extract is selective for transcription of tRNA genes, whereas the TAC is selective for rRNA genes. TAC and the soluble extract respond differently to KCl and Mg2+. The soluble extract is sensitive to heparin, and TAC is resistant. The 2 activities have different temperature optima. This evidence suggests that E. chloroplasts have at least 2 distinct RNA polymerase activities.

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