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Evidence of a different nuclear population in chimeras of regenerated plants from tobacco nicotiana tabacum aneu ploid tissue cultures cyto photometric and statistical analysis


, : Evidence of a different nuclear population in chimeras of regenerated plants from tobacco nicotiana tabacum aneu ploid tissue cultures cyto photometric and statistical analysis. Zeitschrift fuer Pflanzenphysiologie 110(2): 97-106

Leaves of regenerated plants from aneuploid strains can carry chimerae which are chlorophyll deficient. A statistical analysis of the amount of DNA measured by cytophotometry after transformation of mesophyll into a protoplast suspension demonstrated the aneuploidy of these plants and also showed that the chimerae region involves a population of nuclei of different ploidy from those in the rest of the lamina. This latter phenomenon manifests itself in 2 different ways: the average nuclear DNA content of the chimera and the rest of the lamina are significantly different, but the spreads of measured values are similar; the average nuclear DNA are similar but the spreads are significantly different. These chimerae of nuclear origin are, in all cases, the expression in regenerated plants of disturbances suffered by these strains during in vitro culture. The diploid reference (2C), to which the nuclear DNA contents of the material analyzed are compared, corresponds to the average nuclear DNA content of diploid leaf parenchyma of tobacco plantlets from germinated seeds. The reference material and the material studied undergo simultaneously the same experimental treatment.

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Related references

Boucaud, M.-De; Gaultier, J., 1983: Evidence of a different nuclear population in chimerae of regenerated plants from tobacco aneuploid tissue cultures. Cytophotometric and statistical analysis Nicotiana tabacum, chromosomes. Zeitschrift fur Pflanzenphysiologie = International journal of plant physiology10(2): 97-105

Boucaud, M.T. de; Gaultier, J.M., 1983: Evidence of a different nuclear population in chimerae of regenerated plants from tobacco aneuploid tissue cultures. Cytophotometric and statistical analysis. Protoplasts isolated from normal and chlorophyll deficient chimaeric areas of leaves of plants regenerated from two aneuploid tissue culture strains were analysed for nuclear DNA content. Compared with results from a normal diploid 2C control, bot...

Hell, Kg, 1983: Survival of Nicotiana tabacum L. cv. Wisconsin-38 plants regenerated from gamma-irradiated tissue cultures Tobacco. Environmental and experimental botany 23(2): 139-142

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