+ Translate

Evidence of acid phosphatase in the cytoplasm as a distinct entity

, : Evidence of acid phosphatase in the cytoplasm as a distinct entity. Archives of Biochemistry & Biophysics 262(2): 427-438

A study of subcellular acid phosphatase distribution in mammalian tissues shows that isozymes with specific functions are compartmentalized in the cells. The enzyme may be generalized into two types: type A and type B. They are shown by several means to be distinct entities. Type A is confined to the cytoplasm and is inhibited by Cu2+, HCHO, and the coupling agents (for enzyme staining) fast blue RR salt and fast Garnet GBC salt (newly discovered inhibitors), but is insensitive to fluoride and L-(+)-tartrate. Type B is localized in the organelles, presumably lysosomes, in both soluble form and membrane-bound form, with inhibitor sensitivity exactly opposite to that of type A enzyme. Types A and B consist of different sets of isozymes, with sensitivities to inhibitors resembling those observed with the crude extracts of subcellular fractions. Acid phosphatase that exhibits a phosphoryl transfer property was identified as type A enzyme. Type A enzyme has a slightly higher optimal pH and is inhibited by alloxan, whereas for type B, the addition of alloxan broadens the optimal pH to a higher range and elevates the activity at pH 7.4 from negligible to about 30-40% of that obtained under optimal conditions. The alloxan-mediated elevation of type B enzyme activity to this level at the physiological pH may be of considerable significance. Type B enzyme has a high affinity for metabolic intermediates and nucleotides, while type A has an extremely low affinity for these substrates. Cytoplasmic acid phosphatase (type A) is a significnt enzyme population and its activity is not related to the lysosome density in the cells. Type A enzyme in the cytoplasm is thus shown to be an entity distinctly different from type B enzyme in the lysosomes. These findings suggest that the physiological functions of type A acid phosphatase, such as metabolic regulatory processes, merit further studies because of the phosphoryl transfer activity and cytoplasmic localization of the enzyme.

Accession: 005411190

PMID: 3364974

DOI: 10.1016/0003-9861(88)90394-3

Download PDF Full Text: Evidence of acid phosphatase in the cytoplasm as a distinct entity

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Fazekas, F.; Offenbacher, H.; Schmidt, R.; Strasser-Fuchs, S., 1994: MRI of neuromyelitis optica: evidence for a distinct entity. Journal of Neurology, Neurosurgery, and Psychiatry 57(9): 1140-1142

Bertola, D.R.; Antequera, R.; Rodovalho, M.J.; Honjo, R.S.; Albano, L.M.J.; Furquim, I.M.; Oliveira, L.A.; Kim, C.A., 2009: Brachyolmia with amelogenesis imperfecta: further evidence of a distinct entity. Recent onset of head mobility limitations associated with significant stiffness along the vertebral column were the predominating symptomatology in a girl with MURCS association. Occipitoatlantoaxial junction malformation complex and vertebral hyp...

Thorson, K., 1999: Is fibromyalgia a distinct clinical entity? The patient's evidence. In the eyes of a person with fibromyalgia syndrome, their pain and other symptoms are real. By the time a patient seeks medical care for fibromyalgia, they will have probably endured criticism and misunderstandings about the invisible nature of th...

Mäkelä, M.O., 1999: Is fibromyalgia a distinct clinical entity? The epidemiologist's evidence. The key features of fibromyalgia are chronic widespread pain, general distress and enhanced pain sensitivity as revealed by a tender point count. Fibromyalgia could be considered a distinct entity if the key features defined a patient group with a...

Anonymous, 1974: Evidence for a hexose di phosphatase ec 31311 from the cytoplasm of spinach leaves

Egi, Y.; Kawasaki, T., 1977: Purification and properties of an acid phosphatase of Micrococcus denitrificans distinct from thiamine phosphate phosphatase. To determine whether the acid phosphatase in Micrococcus denitrificans participates in hydrolysis of thiamine phosphate in the synthesis of thiamine pyrophosphate, acid phosphatase was purified 280-fold by conventional procedures, which removed th...

Goldblum, J.R.; Rice, T.W.; Richter, J.E., 1996: Vigorous achalasia Pathologic evidence of a distinct disease entity. Gastroenterology 110(4 SUPPL ): A119

Wessely, S.; Hotopf, M., 1999: Is fibromyalgia a distinct clinicl entity? Historical and epidemiological evidence. Bailliere's Best Practice & Research Clinical Rheumatology 13(3): 427-436, Sept

Wessely, S.; Hotopf, M., 1999: Is fibromyalgia a distinct clinical entity? Historical and epidemiological evidence. Most medical specialities have defined medically unexplained syndromes such as fibromyalgia, to categorize patients with prominent but unexplained symptoms. Other such syndromes include irritable bowel syndrome, chronic fatigue syndrome and atypic...

Cohen, M.L., 1999: Is fibromyalgia a distinct clinical entity? The disapproving rheumatologist's evidence. Fibromyalgia syndrome was an attempt to create, for the purposes of investigation, a relatively homogeneous clinical entity out of the clinical phenomena of musculoskeletal pain and tenderness. The attempt has foundered, arising out of circular ar...