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Evidence of an acrosin like enzyme in sea urchin sperm


Developmental Biology 63(2): 299-306
Evidence of an acrosin like enzyme in sea urchin sperm
An enzyme which hydrolyzes benzoyl arginine ethylester was demonstrated in sperm of the sea urchin Strongylocentrotus purpuratus using a sensitive assay employing tritiated substrate. Of the enzyme 80% was exposed but not solubilized by treatment with 30 mM CaCl2 or solubilized egg jelly coat. Enzymatic activity was masked in control sperm not treated with these agents. The exposed enzyme was inhibited by diisopropyl phosphofluoridate (DFP), soybean trypsin inhibitor, or phenylmethane sulfonyl fluoride, suggesting that it was a serine protease and the invertebrate counterpart of vertebrate acrosin. Inhibition of the exposed enzyme with DFP prevented subsequent fertilization.


Accession: 005411219

PMID: 416981



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