EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evidence of antibody dependent killer cell killing by allo activated fc receptor bearing cyto toxic thymus derived lymphocytes


, : Evidence of antibody dependent killer cell killing by allo activated fc receptor bearing cyto toxic thymus derived lymphocytes. Scandinavian Journal of Immunology 6(8): 787-796

Upon in vivo alloactivation of Ig[immunoglobulin]-anti-Ig column-purified [mouse]splenic T [thymus derived] cells in lethally irradiated allogeneic recipients, a variable proportion of donor-derived cytotoxic T lymphocytes (CTL) are able to bind IgG antibody-coated [sheep] erythrocytes through surface Fc receptors (FcR) and form rosettes. The use of fractionation procedures based on the ability of these cells to form rosettes enabled separation of FcR-positive CTL from FcR-negative CTL and examination of the ability of these 2 cell populations to perform as effector cells in direct T-cell-mediated killing and in antibody-dependent cellular cytotoxicity. Direct isolation of the 2 cell populations or deletion of the FcR-positive population by filtration through complexed Ig columns (Ig-anti-Ig) showed both populations to be efficient in direct T-cell mediated cytotoxicity against the relevant target cell. The striking difference between the 2 populations is the exclusive ability of the FcR-positive population to function as effector cells in antibody-dependent cellular cytotoxicity (ADCC). Purification steps before in vivo alloactivation of responding cells for the removal of B [bone marrow derived] cells and FcR-bearing cells with K[antibody dependent killer]-cell activity, followed by procedures to remove phagocytic and adherent cells in the resulting immune spleen cell preparation and, finally, by velocity sedimentation of the rosetting and nonrosetting blasts from the small lymphocyte population, resulted in a population of FcR-positive cells 98% positive for the Thy 1.2 alloantigen. These fractionation steps and immunofluorescence criteria of purity confirm that the ADCC activity within the FcR-positive T-cell population is a property of the CTL itself.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005411254

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Lisowski, F.P.; Ashton, F.; Ormerod, J., 1957: The skeletal remains from the 1952 excavations at Jericho. The excavations of Kenyon at Jericho brought to light human skeletal remains in an extremely friable and powdery state. Represented were 131 adults and 100 non-adults from the Late Chalcolithic to the Middle Bronze Age. One male skull-vault, 2 hum...

Thompson, L.K.; Gaber, B.P.; Liss, H.L.; English, C.K.; Montgomery, M.T., 1994: Use of transposon-generated mutants for the study of polyurethane degradation by pseudomonads. Abstracts of the General Meeting of the American Society for Microbiology 94(0): 469

Lewontin, R. C., 1959: On the anomalous response of Drosophila pseudoobscura to light. Under usual conditions of handling in the laboratory, D. pseudoobscura shows a number of evidences of positive phototaxis. Experiments reported in the present paper show that D. pseudoobscura is indeed negatively phototactic under conditions of lo...

Tran, T.N.; Kosaraju, M.G.; Tamamizu-Kato, S.; Akintunde, O.; Zheng, Y.; Bielicki, J.K.; Pinkerton, K.; Uchida, K.; Lee, Y.Yu.; Narayanaswami, V., 2014: Acrolein modification impairs key functional features of rat apolipoprotein E: identification of modified sites by mass spectrometry. Apolipoprotein E (apoE), an antiatherogenic apolipoprotein, plays a significant role in the metabolism of lipoproteins. It lowers plasma lipid levels by acting as a ligand for the low-density lipoprotein receptor (LDLr) family of proteins, in addi...

Anonymous, 1970: Problems of working hours in connection with the distribution and sale of dairy products. extracts from the congress report III at the 9th Nordic dairy technological congress. Meieriposten 59 (32) 693-695

Anonymous, 2014: ECR 2014, Part A. Insights Into Imaging 5(Suppl 1): 1-133

Rihmer, Z.; Arató, M., 1981: ABO blood groups in manic-depressive patients. The authors compared the ABO blood type distribution pattern of 246 manic-depressive patients to that of 6000 controls. The blood type distribution of all patients did not differ from that of the controls. After subdivision of the patients into su...

Hagopian, G.A.; Ferszt, G.G.; Jacobs, L.A.; McCorkle, R., 1993: Incorporating midmanagement skills in an oncology CNS program. Today's health care system requires that CNSs be prepared to manage care within complex organizations as well as deliver expert care to individuals and their families. The integration of midmanagement preparation with clinical knowledge and s...

Van Der Watt, H.V.H.; Croft, G.J.B., 1993: A kinetic parameter for evaluation of the reactivity of agricultural lime. The "reactivity" and dissolution rate of an aglime in soil is not adequately described by chemical analysis involving neutralization/dissolution under harsh conditions. A method related to dissolution rate at ambient soil pH values shoul...

Mendoca,K.; Marchini,L.C.; Souza,B.deA.; Almeida-Anacleto,D.de; Moreti,A.C.deC.C., 2008: Physicochemical characterization of honey samples produced by Apis mellifera L in a fragment of savannah area in Itirapina county, Sao Paulo State, Brazil. The physicochemical characteristics of honey produced by <i>A. mellifera</i> in a fragment of savannah located in Itirapina, Sao Paulo, Brazil, were studied. Samples were collected monthly from 5 beehives from February to October 2005....