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Evidence of antibody dependent killer cell killing by allo activated fc receptor bearing cyto toxic thymus derived lymphocytes


Scandinavian Journal of Immunology 6(8): 787-796
Evidence of antibody dependent killer cell killing by allo activated fc receptor bearing cyto toxic thymus derived lymphocytes
Upon in vivo alloactivation of Ig[immunoglobulin]-anti-Ig column-purified [mouse]splenic T [thymus derived] cells in lethally irradiated allogeneic recipients, a variable proportion of donor-derived cytotoxic T lymphocytes (CTL) are able to bind IgG antibody-coated [sheep] erythrocytes through surface Fc receptors (FcR) and form rosettes. The use of fractionation procedures based on the ability of these cells to form rosettes enabled separation of FcR-positive CTL from FcR-negative CTL and examination of the ability of these 2 cell populations to perform as effector cells in direct T-cell-mediated killing and in antibody-dependent cellular cytotoxicity. Direct isolation of the 2 cell populations or deletion of the FcR-positive population by filtration through complexed Ig columns (Ig-anti-Ig) showed both populations to be efficient in direct T-cell mediated cytotoxicity against the relevant target cell. The striking difference between the 2 populations is the exclusive ability of the FcR-positive population to function as effector cells in antibody-dependent cellular cytotoxicity (ADCC). Purification steps before in vivo alloactivation of responding cells for the removal of B [bone marrow derived] cells and FcR-bearing cells with K[antibody dependent killer]-cell activity, followed by procedures to remove phagocytic and adherent cells in the resulting immune spleen cell preparation and, finally, by velocity sedimentation of the rosetting and nonrosetting blasts from the small lymphocyte population, resulted in a population of FcR-positive cells 98% positive for the Thy 1.2 alloantigen. These fractionation steps and immunofluorescence criteria of purity confirm that the ADCC activity within the FcR-positive T-cell population is a property of the CTL itself.


Accession: 005411254



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