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Evidence of cytochrome p 450 multiplicity in the midgut of the cluster caterpillar spodoptera litura f


, : Evidence of cytochrome p 450 multiplicity in the midgut of the cluster caterpillar spodoptera litura f. Pesticide Biochemistry & Physiology 29(2): 176-186

Treatment of Spodoptera litura (F.) (the cluster caterpillar)larvae with geraniol (0.4%), pentamethylbenzene (0.2%) .beta.-naphthoflavone (0.2%), and phenobarbital (0.1%) increased cytochrome P-450 content, with geraniol causing a four-fold induction (no differential induction of cytochrome P-450 monooxygenase activity was observed). All treatments, except phenobarbital, increased aldrin epoxidase, ethoxyresorufin-, and ethoxycoumarin-O-deethylase activities. Only geraniol treatment induced epoxidase hydrolase activity. Ethoxyresorufin and ethoxycoumarin-O-deethylase activities were insensitive to carbon monoxide and metyrapine inhibition. In contrast, aldrin epoxidase was sensitive to both inhibitors. Differential inhibition was also seen with .alpha.-naphthoflavone with the O-deethylase activities being more inhibited than aldrin epoxidase at equal inhibitor concentration. Subsequent studies with isosafrole (0.05%) resulted in qualitative changes in midgut microsomal cytochrome P-450 parameters compared to geraniol (0.4%) and control microsomes. Isosafrole treatment induced ethoxyresorufin (.apprx. 20-fold) and ethoxycoumarin-O-deethylase activity while only slightly inducing aldrin epoxidase activity (.apprx. 2-fold). Isosafrole treatments altered the apparent Km for ethoxyresorufin and ethoxycoumarin and increased the proportion of low-spin cytochrome P-450 present in midgut microsomes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of isosafrole-treated microsomes revealed the presence of two protein staining bands of molecular weights 53,500 and 50,500 not observed in control or geraniol-induced microsomes. The results suggest that like S. eridania multiple forms of cytochrome P-450 are present in S. litura midgut microsomes.

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