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Evidence of diffusion artifacts in di amino benzidine immuno cytochemistry revealed during immune electron microscope studies of the early interactions between influenza virus and cells


, : Evidence of diffusion artifacts in di amino benzidine immuno cytochemistry revealed during immune electron microscope studies of the early interactions between influenza virus and cells. Journal of Virological Methods 5(1): 27-34

Anti-hemagglutinin-labeled antibodies were used to search for evidence of influenza entry into cells by fusion of viral and plasma membranes. The plasma membranes of infected African green monkey kidney Vero or chicken fibroblast cells were stained by immunoperoxidase but not by immunoferritin reagents. It is suggested that the staining obtained with the peroxidase conjugate was due to diffusion of the diaminobenzidine reaction product away from the enzymic site. Immunoferritin labeling provided no evidence for entry of influenza by fusion of viral and plasma membranes under conditions of physiological pH.

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