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Evidence of direct thyroid stimulating action of trh by perifusion of rat thyroid fragments


, : Evidence of direct thyroid stimulating action of trh by perifusion of rat thyroid fragments. Endocrinology 116(2): 561-566

TRH stimulation with rat thyroid fragment perfusion was tested. Significant T4 [thyroxine] release was observed for TRH concentrations as low as 1.7 .times. 10-11 M. A dose-response curve was determined. The response was immediate, reaching a peak after the 6th min and continuing for 15 min after the stimulation had ceased. This kinetic pattern is different from the one observed with TSH and with theophylline and suggests that a different mechanism may be involved. TRH seems to be capable of directly stimulating both the secretion of the pituitary hormone (TSH) and the corresponding peripheral gland, like LHRH, which also acts directly on the Leydig cells of the testis.

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Related references

Attali, J.R.; Valensi, P.; Darnis, D.; Perret, G.; Sebaoun, J., 1985: Evidence of direct thyroid-stimulating action of thyrotropin-releasing hormone by perifusion of rat thyroid fragments. The possibility that TRH has a direct thyroid-stimulating action has never been reported. A number of studies have shown a rise in serum concentrations of thyroid hormone after stimulation with TRH, without a rise in TSH secretion. This has led us...

Attali, J.R.; Valensi, P.; Darnis, D.; Weisselberg, C.; Sebaoun, J., 1987: Detection of thyroid-stimulating antibodies in thyroid diseases, employing rat thyroid fragment perifusion. The technique of perifusing rat thyroid fragments was used to investigate the presence of thyroid-stimulating antibodies (TSAb) in the sera of 48 patients. Response to IgG was measured by determining the mean rate of release of T4 (R) during a 30-...

Valensi, P.; Attali, J.R.; Darnis, D.; Sebaoun, J., 1984: Value of the perifusion of fragments of rat thyroid for the dynamic study of thyroid secretion under TSH and theophylline. With the technique of rat thyroid fragment perifusion the kinetics of T4 response to various stimuli can be studied with precision. The response is rapid and brief with theophylline and TSH, which activate membrane adenylcyclase; with the optimal...

Attali, J.R.; Darnis, D.; Valensi, P.; Weisselberg, C.; Sebaoun, J., 1984: Use of the perifusion technique on rat thyroid fragments in the study of thyroid hormone secretion: short-term effects of thyrotrophin, theophylline and glucagon. Perifusion of rat thyroid fragments was performed to study short-term effects of TSH, theophylline and glucagon on thyroid hormone secretion. This technique proved to be relatively convenient and sensitive, and gave reproducible results for at lea...

Attali J.R.; Darnis D.; Valensi P.; Weisselberg C.; Sebaoun J., 1984: Use of the perifusion technique on rat thyroid fragments in the study of thyroid hormone secretion short term effects of thyrotropin theophylline and glucagon. Perifusion of rat thyroid fragments was performed to study short-term effects of TSH, theophylline and glucagon on thyroid hormone secretion. This technique proved to be relatively convenient and sensitive and gave reproducible results for at leas...

Takasu N.; Sato S.; Tsukui T.; Yamada T.; Furihata R.; Makiuchi M., 1974: Inhibitory action of thyroid hormone on the activation of adenyl cyclase cyclic amp system by thyroid stimulating hormone in human thyroid tissues from eu thyroid subjects and thyro toxic patients. Journal of Clinical Endocrinology & Metabolism 39(4): 772-778

Holmes S.; Martin T.J.; Dirmikis S.; Munro D.S., 1978: The in vitro effects of thyroid stimulating hormone and thyroid stimulating immuno globulins on human thyroid membrane adenylate cyclase or cyclic amp accumulation in thyroid slices. Clinical Science & Molecular Medicine 54(2): 35P-36P

Begg, D.J.; Munro, H.N., 1965: Action of thyroid-stimulating hormone on ribonucleic acid synthesis in thyroid slices and in isolated thyroid nuclei. Nature 207(996): 483-485

Mehdi, S.Q.; Kriss, J.P., 1978: Preparation of radio labeled thyroid stimulating immuno globulins by recombining thyroid stimulating immuno globulins heavy chains with iodine 125 labeled light chains direct evidence that the product binds to the membrane thyrotropin receptor and stimulates adenylate cyclase. The biologically active portion of the thyroid-stimulating immunoglobulin (TSI/LATS [long acting thyroid stimulator] molecule is in the Fd portion of the heavy (H) chain. Radioiodination of TSI/LATS by the chloramine T method or contact with the o...

Endo K., 1979: Studies on the radio receptor assay of thyroid stimulating hormone binding of iodine 125 labeled thyroid stimulating hormone to human thyroid receptors and the interaction of long acting thyroid stimulator immuno globulin g. The TSH [thyrotropin] radioreceptor assay system is considered useful in quantitating the hormone and analyzing its mechanism of action. The assay was established and the interaction of abnormal thyroid stimulators in Graves' patients was eva...