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Evidence of histidine phosphorylation in isocitrate lyase from Escherichia coli


, : Evidence of histidine phosphorylation in isocitrate lyase from Escherichia coli. Journal of Biological Chemistry 263(5): 2477-2482

Escherichia coli isocitrate lyase (EC 4.1.3.1.) can be phosphorylated in viro by an ATP-dependent reaction. The enzyme becomes phosphorylated by an endogenous kinase when partially purified sonic extracts are incubated with [.gamma.-32P]ATP. Treatment of isocitrate lyase with dietyl pyrocarbonate, a histidine-modifying reagent, blocked incorporation of [32P]phosphate from [.gamma.-32P]ATP. The isoelectric point of the enzyme was altered by treatment with phosphoramidate, a histidine phosphorylating agent, whch suggests that isocitrate lyase can be phosphorylated at a histidine residue(s). Immunoprecipitated 32P-labeled isocitrate lyase was subjected to alkaline hydrolysis, mixed with chemically synthesized phosphohistidine standards, and analyzed by anion exchange chromatography. Characterization of the phosphoamino acid was based on the demonstration that the 32P-labeled product from alkali-hydrolyzed isocitrate lyase comigrated with synthetic 1-phosphohistidine. In addition, loss of catalytic activity after treatment with potato acid phosphatase indicates that catalytically active isocitrate lyase is the phosphorylated form of the enzyme.

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Accession: 005411468

PMID: 3276689

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