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Evidence of in vitro glycocalyx formation in the bacterial species citrobacter freundii proteus mirabilis and planococcus sp effects of polyosidases






Annales de l'Institut Pasteur Microbiology 138(6): 693-708

Evidence of in vitro glycocalyx formation in the bacterial species citrobacter freundii proteus mirabilis and planococcus sp effects of polyosidases

Three bacterial species of different origin, Citrobacter freundii, Proteus mirabilis and Planococcus sp., formed glycocalyx in vitro and thereby showed that the phenomen does not only occur in vivo contrary to the opinion of many authors. The glycocalyx was produced in the attachment processes used or both extra- and intercellular attachment. The aggregates formed caused large errors in bacterial counts and led to questioning of the very principle of the counting procedures. The glycocalyx was not destroyed by the polyosidases utilized nor by ultrasound treatment. The glycocalyx structure differed only according to the bacterial species of origin and was unchanged by culture media or nutrient deprivation.

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Accession: 005411499



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