EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evidence of muscarinic cholinergic receptor in dog portal vein binding of tritium labeled quinuclidinyl benzilate


, : Evidence of muscarinic cholinergic receptor in dog portal vein binding of tritium labeled quinuclidinyl benzilate. Life Sciences 32(15): 1757-1764

Muscarinic cholinergic receptor sites in dog portal veins were analyzed directly using [3H]quinuclidinyl benzilate (QNB) as a ligand. Specific [3H]QNB binding to crude membrane preparations from the isolated veins was saturable, reversible and of high affinity (Kd = 15.5 .+-. 2.8 pM) with a Bmax [maximum bound concentration] of 110 .+-. 14.7 f[femto]mol/mg protein. Scatchard and Hill plot analyses of the data indicated 1 class of binding sites. From kinetic analysis of the data, association and dissociation rate constants of 1.91 .times. 109 M-1 min-1 and 0.016 min-1, respectively, were calculated. Kd calculated from the equation Kd = K-1/K+1 was 8.3 pM, in good agreement with the Scatchard estimate of Kd (15.5 pM). Specific binding of [3H]QNB was displaced by muscarinic agents. Nicotinic cholinergic agents, .alpha.-bungarotoxin, nicotine and hexamethonium, were ineffective in displacing [3H]QNB binding at 10 .mu.M. These findings provide direct evidence for the existence of muscarinic cholinergic receptors in dog portal veins.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005411587

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Zimmermann, Robert; Schmidt, Stefan; Zingsem, Juergen; Glaser, Anke; Weisbach, Volker; Ruf, Andreas; Eckstein, Reinhold, 2001: Effect of gamma radiation on the in vitro aggregability of WBC-reduced apheresis platelets. BACKGROUND: The effect of gamma radiation on single-donor apheresis platelet concentrates (SDPs) has been elucidated only incompletely. The only existing report on the function of SDPs stored in the irradiated state found a deterioration in the in...

Gaillard, J. M., 1977: Temporal organization of sleep stages in man. By fitting orthogonal polynomials to the cumulated occurrences of sleep stages, it was possible to describe in a simple form their general trend, i.e., their temporal evolution, in the absence of cyclic ultradian variations. Stages 1, 2 and 3 coul...

Meraihi Z.; Lutz O.; Scheftel J.M.; Frey A.; Bach A.C., 1990: Gram negative bacteria sepsis in the rat and tissue lipolytic activity on lct and mct lct based commercial parenteral emulsions. The aim of this study was to evaluate the effect of a gram-negative bacteria sepsis on the activity of the enzymes lipoprotein lipase (LPL) and hepatic lipase (HL), involved in the clearance of circulating triacylglycerol-rich fat particles. Fasti...

Shen, Roh Yu, 2003: Ethanol withdrawal reduces the number of spontaneously active ventral tegmental area dopamine neurons in conscious animals. Withdrawal from chronic ethanol treatment leads to a reduction in the electrical activity in dopamine (DA) neurons in the ventral tegmental area (VTA). However, there is disagreement on how the electrical activity is reduced (i.e., in the number o...

Juo, A. S. R.; Thurow, T. L., 1998: Sustainable technologies for use and conservation of steep lands. Soil and water conservation technologies for steep land farming are reviewed. Studies which illustrate that productivity of some steep lands can be maintained when appropriate technologies are combined with responsible human efforts are described....

Anonymous, 2011: Iowa State engineers study how hills, nearby turbines affect wind energy production. Iowa State University engineers are using wind tunnel tests to study how hills, valleys and the placement of wind turbines affect the performance of wind farms. While the wind power industry has data about offshore turbine performance over flat wa...

Sturman, G.; Freeman, P., 2001: 29th Annual meeting of the European Histamine Research Society, Rome, Italy, May 17-20, 2000. This supplement contains 50 papers presented at a conference. The topics covered were: histamine release from mast cells, basophils and other cell types (10 papers); clinical studies (3); histamine and the central nervous system (8); histamine met...

Nonomura, Kazuo; Tooyama, Ikuo; Abe, Hiromichi; Renda, Tindaro; Erspamer, Vittorio; Kimura, Hirosi, 1993: Immunohistochemical study of postnatal development of deltorphin-I in the rat brain. Neuroscience Research Supplement 0(18): S66

Cordoba, Herman; Fernandez, Margarita; Santos, F.; Oehling, A., 1992: Variations of intracellular histamine basophil levels after treatment with ketotifen. As reported in previous studies, ketotifen modifies the number of basophils in peripheral blood, most likely inhibiting their degranulation, which correlates with the lack of exercise-induced respiratory manifestations. In this study, we attempted...

Darius, A. K.; Mehlhorn, H.; Heydorn, A. O., 2004: Effects of toltrazuril and ponazuril on Hammondia heydorni (syn. Neospora caninum) infections in mice. Mice infected with tachyzoites of Neospora caninum (syn.: Hammondia heydorni) must be pretreated with cortisone in order to show disease symptoms. This indicates the status of an opportunistic agent of disease. Toltrazuril was an effective curativ...