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Evidence of precursors of calcitonin pdn 21 in human milk

, : Evidence of precursors of calcitonin pdn 21 in human milk. Regulatory Peptides 19(1-2): 65-72

Large amounts of immunoreactive PDN-21 (iPDN-21) were found in human milk in concentrations similar to those of immunoreactive calcitonin (iCT): 187 .+-. 73 pM (mean .+-. S.D.) vs 210 .+-. 83 pM (n = 17). Calcitonin (CT) was immunoextracted from milk by means of CT antibodies coupled to Sepharose 4B, and the extracts were gel-chromatographed on Sephadex G-75 after treatment with 6 M guanidine HCl, iCT and iPDN-21 in the fractions were determined with radioimmunoassay. The main part of iCT eluted as high molecular weight forms and these fractions also contained iPDN-21. Enzymatic cleavage of immunoextracted milk CT by trypsin demonstrated that iPDN-21 could be split apart from the high molecular weight forms and be recovered at the position of synthetic human PDN-21 on gel chromatography. iCT was eluted in the region of monomeric CT and as larger forms. Since PDN-21 constitutes the carboxyterminal of preprocalcitonin, our results indicate that human milk contains precursors of calcitonin.

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