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Evidence of transient effect by lignocaine on alveolar macrophage morphology


, : Evidence of transient effect by lignocaine on alveolar macrophage morphology. Journal of Immunological Methods 43(2): 163-168

Alveolar macrophages [MP] were extracted from rat lung by pulmonary lavage. The lavage fluid consisted of Joklik's minimum essential medium alone, containing lignocaine [LC] or LC with the addition of 10% fetal calf serum [FCS]. Although the presence of LC increased the MP yield 3-fold, in the absence of FCS the resulting MP population exhibited gross cytoplasmic vacuolation. The presence of FCS prevented this vacuolar formation. There was no difference in either the osmolarity or pH of the media and although the vacuolation failed to produce changes in a cell viability assay, the presence of the morphological change is obviously undesirable when functional studies are to be carried out.

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Accession: 005411817

PMID: 7264321

DOI: 10.1016/0022-1759(81)90019-3

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