EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evidence suggesting direct oxidation of human erythrocyte membrane sulfhydryls by copper


, : Evidence suggesting direct oxidation of human erythrocyte membrane sulfhydryls by copper. Biochemical and Biophysical Research Communications 82(4): 1294-1299

Cupric ion may directly oxidize the sulfhydryls of human erythrocyte membrane proteins leading to the formation of disulfide links. When packed ghosts were incubated in cupric sulfate (0.3 to 0.7 mM) at pH 8 and electrophoresed on sodium dodecyl sulfate polyacrylamide gels in the absence of dithiothreitol bands 1, 2 (spectrin); 4.2 and 5 (actin) diminished in intensity concomitant with the appearance of high MW material. Band 3 moved to its dimeric position on the gel. Evidence that these crosslinks result from formation of new disulfide links due to direct Cu binding includes: reversal of crosslinking upon addition of dithiothreitol; and blockage of the effect of N-ethylmaleimide, EDTA and mercuric chloride. The effect of Cu was observed under N2, suggesting that it is not related to air oxidation. The crosslinking effect does not require high Cu concentrations if the ghost concentration is low. The possible implication of these results with regard to Cu-induced hemolytic anemias is briefly discussed.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005411891

PMID: 697795

DOI: 10.1016/0006-291x(78)90328-5

PDF Full Text: Evidence suggesting direct oxidation of human erythrocyte membrane sulfhydryls by copper


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Smith, A.; Morgan, W.T., 1978: Transport of heme by hemopexin to the liver evidence for receptor mediated uptake. Carefully defined heme-hemopexin complexes were used to investigate the role of hemopexin in the catabolism of heme in vivo. Uptake of rabbit [59Fe]heme-[125I]hemopexin by rat liver was rapid. The liver-associated 125I reached a maximum 5 minutes...

Anonymous, 2012: Multiple Phosphorylation of Plasma Membrane H+-ATPase. Phosphorylation is an important posttranslational modification of proteins in living cells and primarily serves regulatory purposes. Several methods were employed for isolating phosphopeptides from proteolytically digested plasma membranes of Arab...

Rapalini Augusto E.; Lopez de Luchi Monica, 2000: Paleomagnetism and magnetic fabric of Middle Jurassic dykes from western Patagonia, Argentina. Jurassic volcanism in Patagonia is widespread. Although associated dyke swarms are conspicuous, they have been almost neglected in previous geologic studies of this region. Radiometric, paleomagnetic and magnetic anisotropy studies are reported fr...

Ji, J.; Barbosa, M.A.; Feng, L.; Shen, J., 2004: A novel urethane containing copolymer as a surface modification additive for blood contact materials. Surface modification to develop a biomolecules-presenting surface is of interest, both from a scientific and an industrial point of view. In this research, a penta-block-coupling polymer of warfarin-PEO-MDI-PEO-warfarin was specially designed as t...

Nakade, K.; Handa, H.; Nagata, K., 1998: Promoter structure of the MxA gene that confers resistance to influenza virus. The human MxA protein is one of the interferon-inducible proteins that inhibits multiplication of influenza virus and other viruses. To clarify the control mechanism of its expression, we prepared a series of mutant MxA promoters and identified a...

Holyoake, P.K.; Collins, A.; Donahoo, M.; Lising, R.; Emery, D., 2009: Identifying obstacles to reducing the use of antibiotics to control porcine proliferative enteropathy. Proliferative enteropathy (PE) is an enteric disease of pigs that results in diarrhoea, reduced growth rate, reduced feed conversion efficiency and sometimes death. A survey of 13 pig veterinary practitioners in Australia was conducted to determin...

Kumar, R.; Rathy, S.; Hajare, A.K.; Surase, Y.B.; Dullu, J.; Jadhav, J.S.; Venkataramanan, R.; Chakrabarti, A.; Pandya, M.; Bhateja, P.; Ramkumar, G.; Das, B., 2012: Synthesis and antibacterial activity of a novel series of acylides active against community acquired respiratory pathogens. A novel series of acylides 4 were designed to overcome antibacterial resistance and evaluated for in vitro and in vivo activity. This series of acylides was designed from clarithromycin by changing the substitution on the desosamine nitrogen, foll...

Polston, J.E.; Pritchett, C.E.; Tomasko, J.M.; Rogers, A.M.; Leggio, L.; Thanos, P.K.; Volkow, N.D.; Hajnal, A., 2014: Roux-en-Y gastric bypass increases intravenous ethanol self-administration in dietary obese rats. Roux-en-Y gastric bypass surgery (RYGB) is an effective treatment for severe obesity. Clinical studies however have reported susceptibility to increased alcohol use after RYGB, and preclinical studies have shown increased alcohol intake in obese r...

Aftimos, S.; Asquith, P.; Ashton, F.; Vasilevski, O.; Love, D.R., 2010: Distal 5q deletion with associated parietal foramina. Clinical Dysmorphology 19(1): 43-47

Voloshin, E. I., 2003: Cadmium in soils of the Middle Siberia. The background content of cadmium in soils of the Middle Siberia is 0.09 mg/kg. The spatial and profile distribution of cadmium in soils depends on its biogenic accumulation, soil texture, and element concentration in parent rocks. The existing cr...