EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evidence suggesting that some proteolytic enzymes may cleave only the trans form of the peptide bond


, : Evidence suggesting that some proteolytic enzymes may cleave only the trans form of the peptide bond. Biochemistry 18(1): 43-47

The rates of hydrolysis of glycyl-L-proline and L-phenylalanyl-L-proline, catalyzed by [pig kidney] prolidase, were measured at several temperatures under conditions where a high ratio of prolidase activity to substrate concentration existed. Two well-separated kinetic phases, which can be adequately treated as 2 first-order reactions, were observed for the hydrolysis. The relative amplitudes of the 2 phases are nearly independent of temperature, but strongly dependent on the initial state of protonation of the dipeptides. The amplitude of the slow phase is strictly proportional to the known amount of cis isomer, while the amplitude of the fast phase correlates with the amount of the trans isomer. The relaxation time and activation energy of the slow phase of hydrolysis are in good agreement with the same parameters determined for cis-trans isomerization of the dipeptides, as measured by a pH-jump method for samples not being hydrolyzed. Apparently the slow phase seen for hydrolysis is rate limited by cis-trans isomerization of the X-Pro peptide bond. Thus, this proline-specific protease appears to have an absolute requirement for the trans form of the peptide bond and appears not to cleave the cis form or to cleave it very slowly.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005411906

PMID: 570405

DOI: 10.1021/bi00568a007

PDF Full Text: Evidence suggesting that some proteolytic enzymes may cleave only the trans form of the peptide bond


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Segawa M.; Shiraishi M.; Takebayashi S., 1978: Ultrastructural and biochemical analysis of collagen of human aortic media and vena cava. Journal of Electron Microscopy 27(4): 365

Ipatov, V.S.; Kirikova, L.A., 2001: Characterization of the phytogenic field of Picea abies in Pinetum hylocomiosum forests. Studies were made of the role of individual spruce (Picea abies) trees in the second storey of a middle-aged Scots pine [Pinus sylvestris] forest (density 0.3-0.4) in Russian Karelia. The effect of the spruce trees in transforming the light, tempe...

Hattin Donald E.; Nosow Edmund; Perkins Ronald D.; Stumm Erwin C.; Mound Michael; Utgaard John, 1961: Field excursion to the Falls of the Ohio, Field Trip 9. Pages 295-350 1961

Weber, A.M.; Harrison, T.M.; Steward, D.K., 2013: Schore's regulation theory: maternal-infant interaction in the NICU as a mechanism for reducing the effects of allostatic load on neurodevelopment in premature infants. Premature infants confront numerous physiologic and environmental stressors in the neonatal intensive care unit (NICU) that have the potential to permanently alter their neurodevelopment. Schore’s regulation theory postulates that positi...

Rosin, R. D., 1995: Anastomoses. Mann, C V [Editor], Russell, R C G [Editor], Williams, N S [Editor] Bailey and Love's short practice of surgery, 22nd edition 629-640

Ehle, A.L.; Johnson, P.C., 1977: Rapidly evolving EEG changes in a case of Alzheimer disease. Annals of Neurology 1(6): 593-595

Klingebiel A.; Boltenhaguen C.; Caratini C.; Debyser Y.; Delteil J.R.; Durand B.; Etienne J.; Huc A.; Latouche C.; Millepieds P.; Parra M.; Pujol C.; Sauvan P.; Sommer F., 1975: Stratigraphic, sedimentologic and geochemical studies of sediment cores in the Gulf of Guinea from the cores of the Benin 1971 survey. Bulletin de l'Institut de Geologie du Bassin d'Aquitaine 17): 101-232

Soni, R. C., 1960: A note on natural regeneration of sal. For the success of sal (Shorea robusta) natural regeneration close individual attention and intimate knowledge of local conditions are essential. This is possible if smaller areas are tackled under intensive working with less frequency of changes...

Evans, DL.; Rosenfield, RN., 1985: Migration and mortality of sharp-shinned hawks ringed at Duluth, Minnesota, USA. International Council for Bird Preservation Technical Publication, 311-316 No. 5

Wessler, Susan R., 1998: Transposable elements associated with normal plant genes. Physiologia Plantarum 103(4): 581-586, Aug