EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evidence that a 90 kilodalton phosphoprotein an associated kinase and a specific phosphatase are involved in the regulation of cloudman melanoma cell proliferation by insulin


, : Evidence that a 90 kilodalton phosphoprotein an associated kinase and a specific phosphatase are involved in the regulation of cloudman melanoma cell proliferation by insulin. Proceedings of the National Academy of Sciences of the United States of America 82(4): 1007-1011

Proliferation of wild-type Cloudman S91 melanoma cells is inhibited when insulin is included in the culture medium. Using growth inhibition as a selective marker, variant cell lines were isolated that are either resistant to insulin or dependent upon insulin for growth. The effects of insulin on proliferation were studied by using combined genetic and biochemical approaches. Through a series of genetic hybridization analyses, 3 complementation groups were identified and in general, insulin-sensitivity is dominant to insulin-resistance. Through analyses of in vitro protein phosphorylation reactions, a protein of .simeq. 90 kDa [kilodalton] (pp90) was identified whose phosphorylation is a function of at least one of the complementation groups. Although pp90 is not phosphorylated in extracts of insulin-resistant variants, it is phosphorylated in extracts of insulin-sensitive hybrids formed between complementing resistant variants. Insulin itself exhibits little or no regulation over the phosphorylation of pp90; rather, the ability to phosphorylate pp90 correlates with the ability of cells to respond to insulin. Migration in NaDodSO4[sodium dodecyl sulfate]/polyacrylamide gels, solubility characteristics and divalent cation requirements indicate that pp90 is distinct from the 95-kDa .beta.-subunit of the insulin receptor. Both pp90 and its associated phosphoprotein kinase are found in 30,000 .times. g pellets of sonicated cell lysates, whereas a specific pp90 phosphoprotein phosphatase activity is found in 30,000 .times. g supernatant fractions. Phosphorylation of pp90 occurs at tyrosine and serine residues. Apparently the state of phosphorylation of pp90 is an important determinant in the regulation of cellular proliferation by insulin.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005411971

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Muller, A.; Stellermann, K.; Hartmann, P.; Schrappe, M.; Fatkenheuer, G.; Salzberger, B.; Diehl, V.; Franzen, C., 1999: A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens. To establish a sensitive and specific method for the detection of microsporidia in clinical samples, clinical stool specimens of 104 randomly selected human immunodeficiency virus-infected patients with diarrhoea were studied to compare light micr...

Hovey, R. B.; Cuthbertson, K. E. S.; Birnie, K. A; Robinson, J. W.; Thomas, B. C.; Massfeller, H. F.; Ruether, J. D.; Scott, C., 2012: The Influence of Distress on Knowledge Transfer for Men Newly Diagnosed with Prostate Cancer. The purpose of this inquiry was to evaluate the efficacy of prostate cancer education sessions. Implementation of 3-h patient educational sessions was intended to provide men newly diagnosed with localized prostate cancer, who face difficult and c...

Luz, B.; Kolodny, Y.; Horowitz, M., 1984: Fractionation of oxygen isotopes between mammalian bone-phosphate and environmental drinking water. The 18O of mammalian bone-phosphate varies linearly with 18O of environmental water, but is not in isotopic equilibrium with that water. This situation is explained by a model of 18O in body water in which the important fluxes of exchangeable oxyg...

Lalisse Grundmann, G.; Corman, A.; Chalamet, A., 1983: Simultaneous effect of soil temperature and water content on denitrification. Denitrification in a sandy clay loam in the laboratory ranged from 1.3 to 28.6 g N2O ha-1 day-1, and was closely correlated with the soil temperature and water content. An exponential model was derived which enabled denitrification in the field to...

Anonymous, 1952: Nutrition problems in forest nurseries. 'Partial sterilization' by steam and formalin has given outstanding success in the older nurseries . The application of formalin suitably diluted, at the rate of 250 ml./sq. yd. a month or more before sowing, consistently improved growth...

Bo, N. A., 1961: La presencia en la Argentina de la paloma de ojos desnudos Gymnopelia ceciliae gymnops Chubb. Neotropica, 7: 80

Kellerer A.M., 1971: Event simultaneity in cavities theory of the distortions of energy deposition in proportional counters. Radiation Research: 216-233

Simon, M. R., 1997: Associations between morphological and biochemical markers with the resistance to barley leaf rust (Puccinia hordei Otto). The latent period for the development of barley leaf rust is one of the most important components to the disease. To map genes conferring partial resistance to the disease, recombinant inbred barley lines (F8 generation) of the cross between and 1...

Katsuragi T., 1976: Mechanism of catecholamine release from the uptake 2 area in guinea pig seminal duct. Japanese Journal of Smooth Muscle Research 12(4): 216-217

Ajagbonna, O. P.; Adegunloye, B. J.; Sofola, O. A., 1998: Relaxant effects of isolated rat aorta by Rhaptopetalum coriaceum bark extract. The bark extract of R. coriaceum (collected from Nigeria) was tested for relaxant effects on isolated rat aortic rings precontracted with 10-7 M noradrenaline [norepinephrine]. Relaxation experiments were also conducted on tissues with intact endo...